Document 0861
 DOCN  M9440861
 TI    Severe pulmonary pneumocystosis in simian acquired immunodeficiency
       syndrome induced by simian immunodeficiency virus: its characterization
       by the polymerase-chain-reaction method and failure of experimental
       transmission to immunodeficient animals.
 DT    9404
 AU    Furuta T; Fujita M; Mukai R; Sakakibara I; Sata T; Miki K; Hayami M;
       Kojima S; Yoshikawa Y; Department of Parasitology, University of Tokyo,
       Japan.
 SO    Parasitol Res. 1993;79(8):624-8. Unique Identifier : AIDSLINE
       MED/94126392
 AB    Pneumocystis carinii (Pc) infection was observed in three of five rhesus
       monkeys infected with simian immunodeficiency virus (SIVmac251). They
       showed severe symptoms similar to those associated with human acquired
       immunodeficiency syndrome (AIDS). Histopathology revealed severe
       pulmonary pneumocystosis in one of three Pc-positive monkeys, and
       anti-Pc antibodies were detected in sera from two of the three monkeys.
       Localization of Pc organisms in various organs of the monkeys was
       examined by the polymerase-chain-reaction (PCR) method, and Pc-specific
       bands of DNA amplification were detected in the liver, kidney, spleen,
       adrenal gland, testis, brain, and other organs examined, but no Pc
       organism was found in these organs by histopathologic examination. These
       results suggest that the activation of a latent infection of Pc occurs
       in SIV-infected rhesus monkeys as well as in human AIDS. Experimental
       transmission of Pc derived from a simian was attempted in severe
       combined immunodeficiency (SCID) mice and athymic nude (rnu/rnu, F344)
       rats. These animals were inoculated intranasally with 10(4) Pc cysts,
       but neither histopathologic changes nor Pc organisms were detected in
       SCID mice at 4 months after inoculation or in nude rats at 2 months
       postinoculation, suggesting that simian Pc is species-specific.
 DE    Animal  Antibodies, Fungal/BLOOD  Base Sequence  DNA,
       Fungal/GENETICS/ISOLATION & PURIF  Female  Lymphocytes/MICROBIOLOGY
       Macaca mulatta  Male  Mice  Mice, SCID/MICROBIOLOGY  Molecular Sequence
       Data  Pneumonia, Pneumocystis carinii/ETIOLOGY/PATHOLOGY/*TRANSMISSION
       Polymerase Chain Reaction  Pulmonary Alveoli/PATHOLOGY  Rats  Rats,
       Nude/MICROBIOLOGY  Simian Acquired Immunodeficiency
       Syndrome/*COMPLICATIONS  Support, Non-U.S. Gov't  SIV/ISOLATION & PURIF
       Tissue Distribution  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).