Document 0885
 DOCN  M9440885
 TI    [Detection of HIV-1 proviral DNA in peripheral leukocytes by AMPLICOR
       HIV-1 test kit]
 DT    9404
 AU    Yoshihara N; Fukushima K; Suzuki H; Honda M; Yamazaki S; Fukutake K;
       Meguro T; Yamada K; AIDS Research Center, National Institute of Health,
       Shinjuku-ku,; Tokyo.
 SO    Rinsho Byori. 1993 Dec;41(12):1323-7. Unique Identifier : AIDSLINE
       MED/94125561
 AB    AMPLICOR HIV-1 test kits, which had been developed as an HIV-1 provirus
       detection test by PCR method, have been evaluated for its clinical
       diagnostic application. Sixty-six of HIV-1 antibody positive and 67 of
       HIV-1 antibody negative blood samples derived from hemophiliacs, who had
       received blood products, have been tested by AMPLICOR HIV-1. All of the
       results from AMPLICOR HIV-1 were consistent with those from antibody
       test and clinical aspects. Thirty-nine of HIV-1 antibody positive
       samples have been tested by AMPLICOR HIV-1 and virus isolation (culture
       method). Twelve of 39 (30.8%) were positive by virus isolation, and 39
       of 39 (100%) were positive by AMPLICOR HIV-1. Two of new born infants
       from HIV-1 sero-positive mothers were tested by AMPLICOR HIV-1, and the
       result suggested that the kit would be useful for diagnosis of infants
       from sero-positive mothers. Based on these studies, AMPLICOR HIV-1 is
       considered as useful clinical diagnostic for HIV-1 proviral DNA
       detection.
 DE    Adult  DNA, Viral/*ANALYSIS  English Abstract  Evaluation Studies
       Female  Human  HIV Infections/DIAGNOSIS  HIV-1/*GENETICS/ISOLATION &
       PURIF  Infant, Newborn  Leukocytes/*MICROBIOLOGY  Male  Polymerase Chain
       Reaction  Proviruses/*GENETICS/ISOLATION & PURIF  Reagent Kits,
       Diagnostic/*STANDARDS  JOURNAL ARTICLE

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