Document 0886
 DOCN  M9440886
 TI    [HTLV-I negative adult T cell leukemia; a case report of acute type]
 DT    9404
 AU    Kirito K; Shindou H; Chiba N; Tobinai K; Shimoyama M; Kinoshita T;
       Department of Internal Medicine, Yamanashi Central Hospital.
 SO    Rinsho Ketsueki. 1993 Dec;34(12):1550-5. Unique Identifier : AIDSLINE
       MED/94125547
 AB    We described a case of adult T cell leukemia (ATL) not associated with
       human T-cell leukemia virus type I (HTLV-I), a clinical entity that was
       first reported by Shimoyama et al. A 79-year-old male was admitted with
       anorexia and fever in October, 1989. Physical examination revealed
       marked hepatosplenomegaly and superficial lymphadenopathies.
       Hematological examination revealed marked leukocytosis
       (136,300/microliters) with abnormal lymphoid cells showing highly
       lobulated nuclei. Hypercalcemia (11.2 mg/dl) and elevation of lactic
       dehydrogenase were also recognized. Surface marker analysis showed that
       the abnormal lymphoid cells in the peripheral blood were positive for
       CD2 and CD4 but negative for CD8. Southern blot analysis of the DNA from
       peripheral blood leukemic cells revealed monoclonal rearrangement of
       T-cell receptor beta-chain gene. The clinical and hematological findings
       of the patient were compatible with those of acute type ATL, however,
       serum anti-HTLV-I antibody was negative and HTLV-I proviral DNA was not
       detected in the leukemic cells by Southern blot analysis. Furthermore,
       the polymerase chain reaction showed no integration of the HTLV-I
       proviral DNA in the leukemic cells.
 DE    Aged  Base Sequence  Blotting, Southern  Case Report  DNA,
       Viral/ANALYSIS  English Abstract  Human  *HTLV-I/GENETICS  HTLV-I
       Antibodies/ANALYSIS  Leukemia, T-Cell, Acute/DIAGNOSIS/*MICROBIOLOGY
       Male  Molecular Sequence Data  Polymerase Chain Reaction  JOURNAL
       ARTICLE

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