Document 0915
 DOCN  M9440915
 TI    Human immunodeficiency virus type 1 tat gene enhances human
       papillomavirus early gene expression.
 DT    9404
 AU    Tornesello ML; Buonaguro FM; Beth-Giraldo E; Giraldo G; Istituto
       Nazionale Tumori Fondazione Pascale, Napoli, Italia.
 SO    Intervirology. 1993;36(2):57-64. Unique Identifier : AIDSLINE
       MED/94124226
 AB    To investigate the possible direct/indirect role of Human
       immunodeficiency virus (HIV) as a cofactor in human papillomavirus (HPV)
       oncogenesis, cotransfection experiments were carried out in which a
       recombinant plasmid containing the HPV16 long control region (LCR)
       linked to the chloramphenicol acetyltransferase (CAT) gene was
       cotransfected into cultured cells with a plasmid expressing HIV-1 Tat
       protein. Tat expression efficiency and transactivation activity were
       evaluated in different cell lines by cotransfecting plasmids containing
       the HIV tat gene and HIV LTR-driven CAT-coding sequences. HeLa and CaSki
       cell lines represented the most appropriate recipient cells for
       Tat-directed transactivation of both the HIV LTR and the HPV LCR
       promoters. Furthermore, HIV tat was transfected into HeLa cells
       (containing 10-20 copies per cell of HPV18), and HPV18 E7 protein
       expression was evaluated by a radioimmunoprecipitation assay using
       polyclonal antibodies against the E7 protein. Our results show that the
       Tat protein can transactivate the HPV LCR and increase HPV18 E7
       expression in HeLa cells.
 DE    Gene Expression Regulation, Viral/GENETICS/*PHYSIOLOGY  Gene Products,
       tat/GENETICS/*PHYSIOLOGY  Genes, tat/*PHYSIOLOGY  Hela Cells  Human
       Oncogene Proteins, Viral/GENETICS  Papillomavirus, Human/*GENETICS
       Support, Non-U.S. Gov't  Trans-Activation
       (Genetics)/GENETICS/*PHYSIOLOGY  Transfection  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).