Document 0037
 DOCN  M9460037
 TI    Crystal structure of the principal neutralization site of HIV-1.
 DT    9404
 AU    Ghiara JB; Stura EA; Stanfield RL; Profy AT; Wilson IA; Department of
       Molecular Biology, Scripps Research Institute, La; Jolla, CA 92037.
 SO    Science. 1994 Apr 1;264(5155):82-5. Unique Identifier : AIDSLINE
       MED/94188714
 AB    The crystal structure of a complex between a 24-amino acid peptide from
       the third variable (V3) loop of human immunodeficiency virus-type 1
       (HIV-1) gp 120 and the Fab fragment of a broadly neutralizing antibody
       (59.1) was determined to 3 angstrom resolution. The tip of the V3 loop
       containing the Gly-Pro-Gly-Arg-Ala-Phe sequence adopts a double-turn
       conformation, which may be the basis of its conservation in many HIV-1
       isolates. A complete map of the HIV-1 principal neutralizing determinant
       was constructed by stitching together structures of V3 loop peptides
       bound to 59.1 and to an isolate-specific (MN) neutralizing antibody
       (50.1). Structural conservation of the overlapping epitopes suggests
       that this biologically relevant conformation could be of use in the
       design of synthetic vaccines and drugs to inhibit HIV-1 entry and
       virus-related cellular fusion.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/CHEMISTRY/IMMUNOLOGY
       Antigen-Antibody Complex/*CHEMISTRY/IMMUNOLOGY  Antigen-Antibody
       Reactions  Antigenic Determinants/CHEMISTRY/IMMUNOLOGY  Computer
       Graphics  Crystallography, X-Ray  Hydrogen Bonding  HIV
       Antibodies/*CHEMISTRY/IMMUNOLOGY  HIV Envelope Protein
       gp120/*CHEMISTRY/IMMUNOLOGY  HIV-1/*CHEMISTRY/IMMUNOLOGY
       Immunoglobulins, Fab/*CHEMISTRY/IMMUNOLOGY  Models, Molecular  Molecular
       Sequence Data  Neutralization Tests  Peptide
       Fragments/*CHEMISTRY/IMMUNOLOGY  Protein Conformation  Protein
       Structure, Secondary  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).