Document 0041
 DOCN  M9460041
 TI    Spermatozoa as potential carriers of HIV.
 DT    9404
 AU    Dussaix E; Guetard D; Dauguet C; D'Almeida M; Auer J; Ellrodt A;
       Montagnier L; Auroux M; Laboratoire de Virologie, CHU de Bicetre, Le
       Kremlin-Bicetre,; France.
 SO    Res Virol. 1993 Nov-Dec;144(6):487-95. Unique Identifier : AIDSLINE
       MED/94188579
 AB    In order to investigate the role of germ cells in the sexual
       transmission of immunodeficiency virus (HIV), spermatozoa from healthy
       HIV-seronegative men were incubated in vitro with HIV1. After washing,
       they were cocultured with peripheral blood leukocytes from seronegative
       blood donors. Reverse transcriptase assays and p24 antigen tests were
       performed in culture supernatants. Electron microscopy examination of
       these HIV-incubated spermatozoa was carried out, as well as the search
       for CD4 molecules on their surface. Although virus bound to and seemed
       to enter spermatozoa despite the absence of detectable CD4 epitopes on
       their surface, no replication of HIV was apparent. However, HIV
       particles on the surface of spermatozoa were capable of infecting CD4 T
       lymphocytes. Present results would seem to preclude artificial
       insemination between an HIV-seropositive man and an HIV-seronegative
       woman.
 DE    Adult  Antigens, CD4/ANALYSIS  Human  HIV Infections/TRANSMISSION
       HIV-1/*ISOLATION & PURIF  Male  Spermatozoa/IMMUNOLOGY/*MICROBIOLOGY
       JOURNAL ARTICLE

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