Document 0060
 DOCN  M9460060
 TI    A cellular repressor regulates transcription initiation from the minute
       virus of mice P38 promoter.
 DT    9404
 AU    Krauskopf A; Aloni Y; Department of Molecular Genetics and Virology,
       Weizmann Institute; of Science, Rehovot, Israel.
 SO    Nucleic Acids Res. 1994 Mar 11;22(5):828-34. Unique Identifier :
       AIDSLINE MED/94188136
 AB    We previously reported that the P38 promoter of minute virus of mice
       (MVM) is trans activated by the viral nonstructural protein, NS1,
       through an interaction with a downstream promoter element designated
       DPE. In this communication we report the identification of a distinct
       downstream promoter element which inhibits transcription from the P38
       promoter in vitro, in the absence of the DPE. Removal of 34 bp from the
       region between +95 and +129 downstream from the P38 initiation start
       site relieved inhibition of transcription in whole-cell extract.
       Inhibition was also relieved by the addition, to the transcription
       reaction, of excess DNA fragments which span the putative inhibiting
       element. This indicated the involvement of a trans-acting factor, in
       inhibition of transcription from the P38. Gel retardation experiments
       demonstrated the specific binding of a cellular protein to the
       inhibitory element. This P38 inhibitory element shows spacing and
       orientation dependence as well as promoter specificity. The regulation
       of viral transcription by a cellular repressor may play an important
       role in obtaining a fine temporal order of viral gene expression during
       the course of infection.
 DE    Base Sequence  DNA, Viral  *Gene Expression Regulation, Viral  Hela
       Cells  Human  HIV Long Terminal Repeat/GENETICS  Minute Virus of
       Mice/*GENETICS  Molecular Sequence Data  *Promoter Regions (Genetics)
       Repressor Proteins/*METABOLISM  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Transcription, Genetic  JOURNAL ARTICLE

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