Document 0090
 DOCN  M9460090
 TI    In vivo replicative status and envelope heterogeneity of equine
       infectious anemia virus in an inapparent carrier.
 DT    9404
 AU    Kim CH; Casey JW; Department of Microbiology, Immunology, and
       Parasitology, New; York State College of Veterinary Medicine, Cornell
       University,; Ithaca 14853.
 SO    J Virol. 1994 Apr;68(4):2777-80. Unique Identifier : AIDSLINE
       MED/94187124
 AB    The distribution and replicative status of equine infectious anemia
       virus (EIAV) DNA in the tissues of a well-characterized inapparent
       carrier horse were established by using the PCR technique. The EIAV pol
       region could be amplified in all of the tissues tested, including the
       cerebellum and periventricular tissue, at concentrations approximately
       10(5)-fold less than in the same tissue from an acutely infected horse.
       Further analysis of the EIAV genome, with primer pairs diagnostic for
       sequential stages of reverse transcription, suggests that EIAV DNA in
       the brain, liver, and lymph nodes was incompletely synthesized. The
       products of reverse transcription were found to diminish progressively
       during first-strand synthesis, while products indicative of
       second-strand synthesis were observed only in kidney and spleen DNA
       samples. Sequences specific for different regions of the envelope could
       not be amplified from any of the tissues of the inapparent carrier,
       suggesting that the envelope is highly variable and may be subject to
       extensive drift. Together, the data suggest that low levels of EIAV
       replication persist without causing clinical disease in an inapparent
       carrier.
 DE    Animal  Brain/MICROBIOLOGY  Carrier State/*MICROBIOLOGY  DNA,
       Viral/GENETICS  Equine Infectious Anemia/*MICROBIOLOGY  Genes,
       env/*GENETICS  Genes, gag/GENETICS  Genes, pol/GENETICS  Horses
       Infectious Anemia Virus, Equine/*GROWTH & DEVELOPMENT/GENETICS  Tissue
       Distribution  Virus Replication  JOURNAL ARTICLE

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