Document 0092
 DOCN  M9460092
 TI    An epitope in the V1 domain of the simian immunodeficiency virus (SIV)
       gp120 protein is recognized by CD8+ cytotoxic T lymphocytes from an
       SIV-infected rhesus macaque.
 DT    9404
 AU    Erickson AL; Walker CM; Chiron Corporation, Emeryville, California
       94608.
 SO    J Virol. 1994 Apr;68(4):2756-9. Unique Identifier : AIDSLINE
       MED/94187120
 AB    Cytotoxic T-lymphocyte (CTL) responses against the external envelope
       glycoprotein (gp120) of the simian immunodeficiency virus (SIV) were
       studied in a rhesus macaque infected with SIVmac/239. CD8+ T cells
       enriched from concanavalin A-stimulated peripheral blood mononuclear
       cells lysed autologous target cells infected with recombinant vaccinia
       virus vectors expressing the SIVmac/239 or SIVsm/H4 envelope protein,
       which share approximately 80% identity in amino acid sequence. A CD8+
       CTL line derived by limiting dilution culture of the concanavalin
       A-stimulated lymphocytes was also specific for the envelope proteins of
       both SIV isolates. Mapping studies revealed that this cell line
       recognized an epitope between amino acids 113 and 121 (CNKSETDRW) in the
       V1 domain of gp120. Amino acid substitutions are observed at positions
       116 and 120 among viruses of the SIVsm/mac/human immunodeficiency virus
       type 2 group, and thus synthetic peptides representing these variants
       were tested for the ability to sensitize target cells for lysis by the
       CTL line. Autologous target cells sensitized with a synthetic peptide
       representing the SIVmac/239 sequence were efficiently killed. In
       contrast, recognition of target cells was reduced or abolished when
       peptides representing the amino acid substitutions at position 116 or
       120 of other SIVmac, SIVsm, SIVmne, or SIVstm strains were tested.
       Further studies of CTL responses against this epitope could provide
       insights into mechanisms of variability within the gp120 V1 domain and
       its importance in evasion of immunity in infected or vaccinated monkeys.
 DE    Amino Acid Sequence  Animal  Antigenic Determinants/*IMMUNOLOGY
       Antigens, CD8/*IMMUNOLOGY  Cell Line  Cytotoxicity, Immunologic/DRUG
       EFFECTS  Dose-Response Relationship, Drug  HIV Envelope Protein
       gp120/*IMMUNOLOGY  Macaca  Molecular Sequence Data  Peptide
       Fragments/IMMUNOLOGY/PHARMACOLOGY  Simian Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY  T-Lymphocytes, Cytotoxic/CYTOLOGY/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).