Document 0100
 DOCN  M9460100
 TI    Studies of the conformation-dependent neutralizing epitopes of simian
       immunodeficiency virus envelope protein.
 DT    9404
 AU    Javaherian K; Langlois AJ; Montefiori DC; Kent KA; Ryan KA; Wyman PD;
       Stott J; Bolognesi DP; Murphey-Corb M; Larosa GJ; Repligen Corporation,
       Cambridge, Massachusetts 02139.
 SO    J Virol. 1994 Apr;68(4):2624-31. Unique Identifier : AIDSLINE
       MED/94187104
 AB    It has been shown previously that the major neutralizing epitopes in
       simian immunodeficiency virus (SIV) are discontinuous and conformation
       dependent and that the V3 loop, in contrast to that of human
       immunodeficiency virus (HIV) type 1, does not by itself elicit
       neutralizing antibodies (K. Javaherian et al., Proc. Natl. Acad. Sci.
       USA 89:1418-1422, 1992). We now present data showing that on the basis
       of fractionation of infected macaque sera, protease digestion of the
       envelope, and binding properties of two neutralizing monoclonal
       antibodies to SIV and SIV-HIV chimeric envelope proteins, changes in V3
       can disrupt the conformation-dependent neutralization region. The
       chimeric protein did not produce significant neutralizing antibodies
       against either SIV or HIV. We also report that neutralizing antibodies
       elicited by recombinant SIV envelope proteins of mac251 and B670
       isolates cross-neutralize. Finally, we show that deglycosylation of the
       SIV envelope results in a molecule which binds neither soluble CD4 nor
       the neutralizing monoclonal antibodies being investigated here and does
       not elicit sera with a significant neutralizing titer.
 DE    Acids/PHARMACOLOGY  Amino Acid Sequence  Animal  Antibodies,
       Monoclonal/IMMUNOLOGY  Antibodies, Viral/IMMUNOLOGY  Antigenic
       Determinants/*IMMUNOLOGY  Antigens, CD4/METABOLISM  Antigens,
       Viral/*IMMUNOLOGY  Chimeric Proteins/IMMUNOLOGY  Cross Reactions
       Glycosylation  Guinea Pigs  HIV Envelope Protein
       gp120/GENETICS/IMMUNOLOGY  Macaca  Molecular Sequence Data
       Neutralization Tests  Peptide Fragments/IMMUNOLOGY  Protein Binding
       Protein Conformation  Protein Processing, Post-Translational
       Retroviridae Proteins/GENETICS/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.
       SIV/*IMMUNOLOGY  Viral Envelope Proteins/GENETICS/*IMMUNOLOGY  JOURNAL
       ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).