Document 0106
 DOCN  M9460106
 TI    Dynamics of molecular parameters of human immunodeficiency virus type 1
       activity in vivo.
 DT    9404
 AU    Bagnarelli P; Valenza A; Menzo S; Manzin A; Scalise G; Varaldo PE;
       Clementi M; Institute of Microbiology, University of Ancona, Italy.
 SO    J Virol. 1994 Apr;68(4):2495-502. Unique Identifier : AIDSLINE
       MED/94187091
 AB    The dynamics of viral activity during different phases of human
       immunodeficiency virus type 1 (HIV-1) infection were investigated by
       competitive PCR methods. In particular, we studied the time course of
       three quantitative molecular parameters of viral activity (genomic RNA
       copy number in plasma and provirus and late HIV-1 transcript molecule
       copy numbers in peripheral blood CD4+ T lymphocytes) in untreated
       patients and patients treated with specific anti-HIV-1 compounds. The
       results shown here indicate that direct RNA parameters are quantitative
       molecular indices sensitive enough to be used for a more accurate
       evaluation of the natural history of this infection and that an indirect
       parameter, the mean transcriptional activity for each provirus in CD4+ T
       lymphocytes, may be important in studying this infection in vivo at the
       molecular level. A dramatic decrease of the indices was evident at
       seroconversion, but the quantitative values were virtually stable
       throughout the time the untreated patients were studied during the
       clinical latency phase. Furthermore, the results indicate that an early
       response to antiretroviral compounds is detected in most subjects as a
       decrease in the viral activity level.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY/IMMUNOLOGY/
       MICROBIOLOGY  Adult  Didanosine/THERAPEUTIC USE  Female  Human  HIV
       Infections/DRUG THERAPY/IMMUNOLOGY/*MICROBIOLOGY  HIV
       Seropositivity/DRUG THERAPY/IMMUNOLOGY/MICROBIOLOGY  HIV-1/*GROWTH &
       DEVELOPMENT/GENETICS/IMMUNOLOGY  Leukocytes, Mononuclear/MICROBIOLOGY
       Male  Middle Age  Polymerase Chain Reaction  Proviruses  RNA,
       Viral/BLOOD  Support, Non-U.S. Gov't  Time Factors  Transcription,
       Genetic  T4 Lymphocytes/MICROBIOLOGY  Zidovudine/THERAPEUTIC USE
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).