Document 0115
 DOCN  M9460115
 TI    Human immunodeficiency virus type 1 Rev-responsive element RNA binds to
       host cell-specific proteins.
 DT    9404
 AU    Shukla RR; Kimmel PL; Kumar A; Department of Biochemistry and Molecular
       Biology, George; Washington University Medical Center, Washington, D.C.
       20037.
 SO    J Virol. 1994 Apr;68(4):2224-9. Unique Identifier : AIDSLINE
       MED/94187062
 AB    RNase protection-gel retention studies show human host cell-specific
       ribonucleoprotein complexes with human immunodeficiency virus type 1
       Rev-responsive element (RRE) RNA. Nuclear proteins from rodent or murine
       cells appear to lack the ability to form these complexes. Human-mouse
       somatic cell hybrids retaining a single human chromosome, either 6 or
       12, form the RRE-nuclear-protein complexes. One of the complexes
       requires the entire RRE RNA, while the other needs RRE RNA stem-loops 1
       and 2 only. Two major proteins with molecular masses of 120 and 62 kDa
       specifically bind to RRE RNA. Rodent cells (CHO) either lack or contain
       small amounts of these RRE-binding proteins.
 DE    Animal  Comparative Study  Cross-Linking Reagents  *Genes, env  Hela
       Cells  Human  HIV-1/*METABOLISM  Mice  Models, Molecular  Nucleic Acid
       Conformation  Protein Binding  *Regulatory Sequences, Nucleic Acid
       Ribonucleoproteins/METABOLISM/RADIATION EFFECTS  RNA-Binding
       Proteins/*METABOLISM  RNA, Viral/*METABOLISM  Species Specificity
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Ultraviolet Rays
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).