Document 0140
 DOCN  M9460140
 TI    A phenotypic study of CD8+ lymphocyte subsets in infants using
       three-color flow cytometry.
 DT    9404
 AU    Jennings C; Rich K; Siegel JN; Landay A; Department of
       Immunology/Microbiology, Rush-Presbyterian-St.; Luke's Medical Center,
       Chicago, Illinois 60612.
 SO    Clin Immunol Immunopathol. 1994 Apr;71(1):8-13. Unique Identifier :
       AIDSLINE MED/94185343
 AB    Flow cytometry is a powerful tool for the multiparametric evaluation of
       cell surface phenotype in patients with HIV disease. Many cell surface
       molecules can be evaluated by three-color flow cytometry and the markers
       correlated with functional activity. It has recently been recognized in
       adults that the CD8 cell is an important lymphocyte subset in HIV
       disease that correlates with disease outcome, but there is little
       information about CD8 subsets in infants. Therefore, we studied infants
       born to HIV-infected mothers and those born to uninfected mothers. No
       significant differences were seen in phenotypic markers of activation
       (CD38, HLA-DR), maturation (CD45RO, CD45RA), and function (CD28) between
       uninfected infants born to HIV infected or uninfected mothers. In
       HIV-infected infants, a substantial increase in CD8+ CD38+ HLA-DR+
       expression was seen. In addition, we found that there was a significant
       increase in the CD8+ CD45RO+ CD45RA- subset which is characteristic of
       the memory phenotype. Finally, evaluation of CD28 (costimulatory
       molecule involved in T cell activation), which is expressed on almost
       all CD8 cells at birth, showed that this population was significantly
       reduced in infected infants. These studies suggest that three-color flow
       cytometry is a powerful tool for evaluating phenotypic changes in
       lymphocyte subsets and enhancing our understanding of the pathobiology
       of HIV disease.
 DE    Antigens, CD8/*ANALYSIS/GENETICS  Flow Cytometry/*METHODS  Human  HIV
       Infections/DIAGNOSIS  Infant  Lymphocyte Subsets/*IMMUNOLOGY  Lymphocyte
       Transformation/IMMUNOLOGY  Phenotype  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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