Document 0142
 DOCN  M9460142
 TI    Subpopulations of T and B cells in perinatally HIV-infected and
       noninfected age-matched children compared with those in adults.
 DT    9404
 AU    Ibegbu C; Spira TJ; Nesheim S; Mendez H; Lee F; Polliotti B; Caba J;
       Nahmias A; Department of Pediatrics, Emory University School of
       Medicine,; Atlanta, Georgia 30303.
 SO    Clin Immunol Immunopathol. 1994 Apr;71(1):27-32. Unique Identifier :
       AIDSLINE MED/94185335
 AB    Peripheral blood mononuclear cells were quantified for the subsets of
       CD4, CD8, and CD19 lymphocytes by using CD45RA (2H4), CD29(4B4), CD57,
       CD5, CD10, Leu8, HLA-DR, and TCR gamma delta-1 monoclonal antibodies and
       dual color immunofluorescence. A comparative analysis of lymphocyte
       subpopulations was made among 52 HIV-infected and 50 age-matched control
       children and 30 HIV-seropositive and 27 negative control adults. A
       significant decrease in the CD4+CD45RA+ naive cells was much more marked
       in HIV-infected children than in HIV-infected adults. A significant
       percentage increase in the CD4+CD29+ memory cells was observed in
       HIV-infected children but not in infected adults; however, the absolute
       numbers were usually decreased in all age groups. The mean percentage
       and absolute numbers of CD4+CD7+ and CD4+Leu8+ cells were decreased in
       HIV-infected children, although usually not significantly. The CD3+TCR
       gamma delta-1+ did not show any change in the infected children tested.
       The mean percentage and absolute number of the CD8+HLA-DR+ cells
       increased significantly in HIV-infected persons of all ages. The
       CD8+CD57+ cells were increased in percentage and absolute number in
       HIV-infected children ages 1-4 and 4-8 years. In the adults, no change
       was noted in either the percentage or absolute number of CD19+CD5+ B
       cells, a finding similar to that noted in HIV-infected children above 1
       year of age. Although adults showed a significant decrease in both
       percentage and numbers of CD5- B cells, an increase was noted in the 7-
       to 12-month-old HIV-infected children. The CD19+CD10+ cells showed a
       slight but significant decrease in the youngest age group and a
       significant increase in the older age groups of HIV-infected children.
       These findings indicate that several lymphocyte subpopulations are
       altered differentially during HIV infection in children of varying ages
       and in adults.
 DE    Adult  Aging/IMMUNOLOGY  Antigens, CD/ANALYSIS  Antigens, CD8/ANALYSIS
       Antigens, Differentiation, B-Lymphocyte/ANALYSIS  *B-Lymphocyte
       Subsets/IMMUNOLOGY  Child  Child, Preschool  Comparative Study  Human
       HIV Infections/CONGENITAL/*ETIOLOGY/IMMUNOLOGY  Immunologic Memory
       Infant  Membrane Metallo-Endopeptidase/ANALYSIS  Support, U.S. Gov't,
       P.H.S.  *T-Lymphocyte Subsets/IMMUNOLOGY  T4 Lymphocytes/CLASSIFICATION
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).