Document 0144
 DOCN  M9460144
 TI    T cell activation in pediatric AIDS pathogenesis: three-color
       immunophenotyping.
 DT    9404
 AU    Plaeger-Marshall S; Isacescu V; O'Rourke S; Bertolli J; Bryson YJ;
       Stiehm ER; Department of Pediatrics, UCLA School of Medicine 90024.
 SO    Clin Immunol Immunopathol. 1994 Apr;71(1):19-26. Unique Identifier :
       AIDSLINE MED/94185333
 AB    Immune activation is an important component of HIV disease in adults
       that may reflect a protective host response and/or be a component of
       immunopathogenesis. The goals of this study were to gain understanding
       of T cell activation in pediatric HIV disease, to assess the usefulness
       of T cell activation markers as surrogates for disease progression
       and/or early identification of infection in infants at risk, and to
       determine any advantages of three- compared to two-color flow cytometric
       immunophenotyping for the above assessments. We examined the expression
       of cell-surface activation antigens on the CD4 and CD8 T cells of 26
       HIV-infected and 40 HIV-seronegative age-matched control children.
       Compared with controls, HIV-infected children showed a slight but not
       significant decrease in the proportion of CD4 cells that coexpressed
       CD45RA and L-selectin (mean of 83 vs 75% for < 2 years of age, 76 vs 62%
       for 2-3 years, 64 vs 56% for > or = 4 years). CD4 cells coexpressing
       CD38 and HLA-DR were significantly increased in HIV+ children (mean of 2
       vs 6% for < 2 years of age, 3 vs 11% for 2-3 years, 2 vs 8% for > or = 4
       years). There was a striking and significant increase in the proportion
       of CD8 cells coexpressing CD38 and HLA-DR (mean of 5 vs. 25% for < 2
       years, 10 vs 41% for 2-3 years, 6 vs 31% for > or = 4 years); this
       double positive population of CD8 cells included cells that were
       approximately 1 log brighter for the expression of CD38 than for that of
       CD38 single-positive cells. There was a significant reduction in CD45RA+
       CD8 cells (means of 92 vs 71% for < 2 years of age, 88 vs 50% for 2-3
       years, 80 vs 57% for > or = 4 years) and an increase in CD57+ CD8 cells
       (mean of 4 vs 8% for < 2 years of age, 8 vs 22% for 2-3 years, 19 vs 31%
       for > or = 4 years) in HIV+ children. The inclusion of CD3 as an anchor
       marker for CD8 cell subsets to limit the analysis to CD3+ CD8 cells did
       not substantially alter the data nor enhance the differences between
       infected and control children compared with the analysis of all CD8
       cells.(ABSTRACT TRUNCATED AT 400 WORDS)
 DE    Acquired Immunodeficiency Syndrome/*ETIOLOGY/*IMMUNOLOGY
       Aging/IMMUNOLOGY  Antigens, CD/ANALYSIS  Antigens, CD3/ANALYSIS
       Antigens, CD4/ANALYSIS  Antigens, CD8/ANALYSIS  Antigens,
       Differentiation, T-Lymphocyte/ANALYSIS  Child  Child, Preschool
       Comparative Study  Human  HIV Infections/PHYSIOPATHOLOGY  HLA-DR
       Antigens/ANALYSIS  Immunophenotyping/METHODS  Infant  Lymphocyte
       Transformation  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocyte Subsets/IMMUNOLOGY  T-Lymphocytes/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).