Document 0171
 DOCN  M9460171
 TI    Effect of different human immunodeficiency virus type-1 (HIV-1) isolates
       on long-term bone marrow haemopoiesis.
 DT    9404
 AU    Cen D; Zauli G; Szarnicki R; Davis BR; Geraldine Brush Cancer Research
       Institute, Medical Research; Institute, San Francisco, CA 94115.
 SO    Br J Haematol. 1993 Nov;85(3):596-602. Unique Identifier : AIDSLINE
       MED/94183738
 AB    Haemopoietic cytopenias are a frequent occurrence in human
       immunodeficiency virus type-1 (HIV-1) induced disease. In order to
       examine the possible direct inhibition of marrow haemopoiesis by HIV-1,
       we have investigated the effect of HIV-1 infection on myelopoiesis in
       long-term bone marrow cultures. In vitro exposure of normal marrow
       cultures to three different lymphocytotropic HIV-1 isolates resulted in
       productive infection, as demonstrated by a progressive increase of gag
       p24 antigen. In these experiments, ICR-3 isolate, but not LAV' or NL4-3
       isolates, accelerated the loss of non-adherent cells. A differential
       ability of these HIV-1 isolates to suppress myelopoiesis was confirmed
       in long-term cultures in which virus was added continuously. In these
       cultures, ICR-3, and to a lesser extent also NL4-3, but not LAV',
       induced a progressive decrease in the number of total non-adherent cells
       as well as non-adherent colony forming units-granulocyte/macrophage
       (CFU-GM). Furthermore, exposure of normal purified CD34+ cells to ICR-3
       induced defects in their ability to form haemopoietic colonies; this
       inhibitory effect was significantly relieved by pretreatment of ICR-3
       with an anti-gp120 antibody. Similar exposure of CD34+ cells to LAV' and
       NL4-3 induced no such defects. These data indicate that some HIV-1
       isolates can impair bone marrow haemopoiesis in a dose-dependent
       fashion, acting, at least in part, at the level of haemopoietic
       stem/progenitor cells.
 DE    Antigens, CD/ANALYSIS  Bone Marrow/*MICROBIOLOGY  Cells, Cultured
       Colony-Forming Units Assay  *Hematopoiesis  Hematopoietic Stem
       Cells/PATHOLOGY  Human  HIV Infections/*PHYSIOPATHOLOGY  *HIV-1
       Support, U.S. Gov't, P.H.S.  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).