Document 0247
 DOCN  M9460247
 TI    Detection of rectal antibodies to HIV-1 by a sensitive chemiluminescent
       western blot immunodetection method.
 DT    9404
 AU    Mohamed OA; Ashley R; Goldstein A; McElrath J; Dalessio J; Corey L;
       Department of Epidemiology, School of Public Health, University; of
       Washington, Seattle.
 SO    J Acquir Immune Defic Syndr. 1994 Apr;7(4):375-80. Unique Identifier :
       AIDSLINE MED/94180312
 AB    Western blot with a time-dependent enhanced chemiluminescence
       immunodetection method (ECL-WB) was shown to be 100-fold more sensitive
       than standard commercial colorimetric Western blots (WB) for detecting
       serum IgG to human immunodeficiency virus type 1 (HIV-1). ECL-WB was
       then used to test rectal secretions from 15 HIV-1 infected subjects
       (HIV+) and 7 uninfected subjects (HIV-) to document local IgG, IgA, and
       secretory component-associated immunoglobulin (SC-Ig) to HIV-1 proteins.
       Fourteen of 15 HIV+ subjects had rectal IgA to at least 1 HIV-1 protein,
       most often to gp41 (80%) or p24 (60%) and 14 (93%) had IgG to gp160,
       gp120, or gp41. Of seven HIV- subjects, none had detectable bands to
       HIV-1 proteins. SC-Ig to HIV-1 proteins was detected in all five rectal
       samples tested. However, the antibody profiles differed from those of
       rectal IgA, suggesting more than one source of rectal IgA to HIV. ECL-WB
       requires individual optimization and interpretation for each specimen as
       well as expensive reagents and is, therefore, not currently applicable
       to screening assays. However, the method offers promise as a sensitive
       method to characterize low-level immune responses (IgG, IgA, and SC-Ig)
       to HIV-1 proteins at local sites such as rectal mucosae.
 DE    Blotting, Western  Chemiluminescence  Comparative Study  Homosexuality
       Human  HIV Antibodies/*ANALYSIS  HIV Seropositivity/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  IgA, Secretory/ANALYSIS  IgG/ANALYSIS  Intestinal
       Mucosa/IMMUNOLOGY/SECRETION  Male  Rectum/*IMMUNOLOGY/SECRETION
       Sensitivity and Specificity  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

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