Document 0249
 DOCN  M9460249
 TI    Diurnal and short-term stability of HIV virus load as measured by gene
       amplification.
 DT    9404
 AU    Holodniy M; Mole L; Winters M; Merigan TC; AIDS Research Center,
       Department of Veterans Affairs Medical; Center, Palo Alto, CA 94304.
 SO    J Acquir Immune Defic Syndr. 1994 Apr;7(4):363-8. Unique Identifier :
       AIDSLINE MED/94180310
 AB    To determine whether human immunodeficiency virus (HIV) viral load has
       short term stability, eight clinically stable subjects infected with HIV
       and having CD4 counts ranging between 10-600/mm3, had blood samples
       taken at 0800 and 1700 on 3 consecutive days and then weekly at 0800 for
       1 month (8-10 observations/subject). Plasma HIV RNA, peripheral blood
       mononuclear cell (PBMC) proviral DNA, serum p24 antigen levels, and
       mononuclear cell subsets were measured at each time point. Mean plasma
       HIV RNA, PBMC HIV DNA, and p24 antigen [both regular and immune complex
       dissociated (ICD)] levels did not change significantly between mornings
       and afternoons or on successive days or weeks. CD4+, CD8+, and CD56+
       number demonstrated a diurnal variation in those subjects with > 200 CD4
       cells/mm3. We conclude that HIV viral load demonstrates short-term
       stability in clinically stable subjects. This stability has important
       implications for monitoring HIV disease progression or antiretroviral
       therapy.
 DE    Adult  Analysis of Variance  *Circadian Rhythm  DNA, Viral/*BLOOD  Human
       HIV/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF  HIV Core Protein p24/BLOOD
       HIV Infections/*MICROBIOLOGY  Leukocyte Count  Lymphocyte Subsets
       Middle Age  Polymerase Chain Reaction  Proviruses/GENETICS/ISOLATION &
       PURIF  RNA, Viral/*BLOOD  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  T4 Lymphocytes  Viremia/MICROBIOLOGY  JOURNAL ARTICLE

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