Document 0271
 DOCN  M9460271
 TI    Human immunodeficiency virus type 1 causes productive infection of
       macrophages in primary placental cell cultures.
 DT    9404
 AU    McGann KA; Collman R; Kolson DL; Gonzalez-Scarano F; Coukos G;
       Coutifaris C; Strauss JF; Nathanson N; Department of Pediatrics,
       University of Pennsylvania Medical; Center, Philadelphia.
 SO    J Infect Dis. 1994 Apr;169(4):746-53. Unique Identifier : AIDSLINE
       MED/94179882
 AB    To characterize the role of the placenta in vertical transmission of
       human immunodeficiency virus type 1 (HIV-1), the susceptibility of
       primary human placental cultures and of transformed trophoblast cell
       lines to infection by several HIV-1 isolates was examined. Placental
       cultures supported the replication of all strains tested, including
       lymphocyte-, macrophage-, and amphotropic isolates. All viruses
       replicated to modest levels, with production of both viral antigen and
       infectious virus in the culture supernatants. Placental cells
       demonstrated a pattern of permissiveness for HIV-1 isolates distinct
       from that seen with lymphocytes, blood-derived macrophages, or T cell
       lines. Immunofluorescent staining showed that 5%-10% of the cultured
       placental cells expressed viral antigens, and double labeling revealed
       that the HIV-positive cells were macrophages not trophoblasts. None of
       the trophoblast cell line (JEG-3, Jar, BeWo, HP-W1) could be infected by
       HIV. These results support the hypothesis that infection of the placenta
       could play a role in maternofetal transmission of HIV-1 and suggest that
       the placental macrophage is likely to be the primary cell type
       responsible.
 DE    Cell Line, Transformed  Cells, Cultured  Choriocarcinoma  Female
       Fluorescent Antibody Technique  Hela Cells  Human  HIV
       Antigens/BIOSYNTHESIS  HIV-1/*PHYSIOLOGY  Macrophages/*MICROBIOLOGY
       Placenta/CYTOLOGY/*MICROBIOLOGY  Pregnancy  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Trophoblast/MICROBIOLOGY  Tumor Cells,
       Cultured  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).