Document 0348
 DOCN  M9460348
 TI    Binding of an HIV Rev peptide to Rev responsive element RNA induces
       formation of purine-purine base pairs.
 DT    9404
 AU    Battiste JL; Tan R; Frankel AD; Williamson JR; Department of Chemistry,
       Massachusetts Institute of Technology,; Cambridge 02139.
 SO    Biochemistry. 1994 Mar 15;33(10):2741-7. Unique Identifier : AIDSLINE
       MED/94176462
 AB    The Rev responsive element (RRE) is an RNA secondary structural element
       within the env gene of HIV and is the binding site for the viral Rev
       protein. Formation of the Rev-RRE complex is involved in regulation of
       splicing and transport of mRNA from the nucleus. To understand the
       structural basis for the specific recognition of RRE by Rev, we have
       studied a model system for this interaction using NMR. We have obtained
       a specific 1:1 complex between an RNA derived from stem IIB of RRE,
       which contains the highest affinity Rev binding site, and a modified
       Rev34-50 peptide, which binds the RRE as an alpha-helix [Tan, R., et al.
       (1993) Cell 73, 1031-1040]. Binding of the peptide was accompanied by a
       conformational change in the RNA, which resulted in the formation of
       additional base pairs not present in the free RNA. Two of these induced
       base pairs are purine-purine pairs within the internal loop of RRE,
       which had been previously proposed on the basis of biochemical
       experiments [Bartel, D.P., et al. (1991) Cell 67, 529-536]. The
       formation of non-Watson-Crick base pairs, interactions in the major
       groove, and protein-induced conformational changes may prove to be
       common characteristics of RNA recognition of proteins.
 DE    Amino Acid Sequence  *Base Composition  Base Sequence  Binding Sites
       Gene Products, rev/CHEMISTRY/*METABOLISM  Hydrogen Bonding
       HIV/GENETICS/*METABOLISM  Molecular Sequence Data  Nuclear Magnetic
       Resonance  Nucleic Acid Conformation  Peptide
       Fragments/CHEMISTRY/CHEMICAL SYNTHESIS/METABOLISM  Protein Binding
       Protein Conformation  Purines  RNA, Viral/CHEMISTRY/*METABOLISM
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).