Document 0386
 DOCN  M9460386
 TI    Detection of HTLV-I pX gene by polymerase chain reaction using newly
       designed primers.
 DT    9404
 AU    Imajo K; Shinagawa K; Tada S; Tsubota T; Kimura I; Second Department of
       Internal Medicine, Okayama University; Medical School, Japan.
 SO    Acta Med Okayama. 1993 Dec;47(6):355-61. Unique Identifier : AIDSLINE
       MED/94175023
 AB    Newly designed oligonucleotide primers, KI-7 and KI-8 for the human T
       cell lymphotropic virus type I (HTLV-I) pX gene were synthesized using
       an automated DNA synthesizer. Previously known HTLV-I-infected cell
       lines, MT-1 and MT-2, were used as positive controls and
       HTLV-I-uninfected cell lines, Molt-4, SBC-3, ABC-1, and EBC-1, as
       negative controls. Peripheral blood mononuclear cells from 17 patients
       with anti-HTLV-I antibody and 10 healthy individuals without anti-HTLV-I
       antibody were studied by polymerase chain reaction (PCR) with KI-7 and
       KI-8. All DNA samples from HTLV-I-infected cell lines and 17 patients
       with anti-HTLV-I antibodies showed positive signals of the HTLV-I pX
       gene. None of the DNA samples from HTLV-I-uninfected cell lines or 10
       healthy individuals showed positive signals. When serially diluted DNA
       of MT-2 cells were amplified by 35 cycles of PCR, the detection limit of
       the pX gene by using the primer pairs was DNA from about 1.5 MT-2 cells.
       Specificity and detectable capacity of primer pairs, KI-7 and KI-8 were
       confirmed to be enough to use for the diagnosis of HTLV-I infection.
 DE    Base Sequence  Blotting, Southern  Cell Line  DNA, Viral/ANALYSIS
       *Genes, pX  Human  HTLV-I/*GENETICS  HTLV-I Antibodies/ANALYSIS
       Molecular Sequence Data  *Oligonucleotide Probes/GENETICS  Polymerase
       Chain Reaction/*METHODS  JOURNAL ARTICLE

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