Document 0396
 DOCN  M9460396
 TI    Anti-idiotypic antibodies to the third variable domain of gp120 induce
       an anti-HIV-1 antibody response in mice.
 DT    9404
 AU    Boudet F; Theze J; Zouali M; Immunogenetique Cellulaire, Institut
       Pasteur, Paris, France.
 SO    Virology. 1994 Apr;200(1):176-88. Unique Identifier : AIDSLINE
       MED/94174715
 AB    We tested the potential of anti-idiotypic antibodies to function as
       molecular mimics of a neutralizing epitope of the human immunodeficiency
       virus (HIV). Three monoclonal antibodies to the third variable domain
       (V3) of gp120 from the HIV-1LAI isolate were raised in a BALB/c mouse.
       They bound gp120LAI with high affinity (K congruent to 10(-11) M),
       immunoprecipitated viral gp120LAI, recognized HIVLAI-infected cells by
       immunofluorescence and neutralized HIVLAI infection in vitro. Mice and
       rabbits were immunized against each one of these antibodies, and one
       monoclonal and two polyclonal anti-idiotypic reagents were selected for
       further study. The three anti-idiotypes recognized binding site-related
       idiotopes that were not present on other V3-specific mouse sera or
       monoclonal antibodies. Groups of mice and rabbits were immunized against
       these anti-idiotypes presented in a homopolymerized form, conjugated to
       a protein carrier, or in an uncoupled form. In the absence of antigen,
       mice that received polyclonal anti-idiotypes mounted an antibody
       response to gp120LAI, but not to V3-deleted gp120LAI recombinant
       proteins. Unlike the three nominal mAbs, the induced antibodies also
       reacted with gp120 from the divergent HIV strain SF2. The isotypic
       distribution of the anti-idiotype-induced antibodies and their temporal
       evolution resemble the restricted pattern and the rapid rise of the
       antibody response obtained after administration of recombinant gp120LAI
       to mice. These data thus present evidence that anti-idiotypes raised
       against HIV-specific antibodies may substitute viral antigens for
       induction of a humoral immune response to HIV. They also suggest that
       the HIV epitope variability may be, in part, overcome with the use of
       anti-idiotypic reagents.
 DE    Animal  Antibodies, Anti-Idiotypic/*IMMUNOLOGY  Antibodies,
       Monoclonal/IMMUNOLOGY  Antibody Specificity  Antigenic
       Determinants/IMMUNOLOGY  Binding, Competitive  Female  HIV
       Antibodies/*BIOSYNTHESIS  HIV Envelope Protein
       gp120/GENETICS/*IMMUNOLOGY  HIV-1/GENETICS/*IMMUNOLOGY  Immunization
       Immunoglobulin Isotypes  Mice  Mice, Inbred BALB C  Neutralization Tests
       Rabbits  Recombinant Proteins/IMMUNOLOGY  Support, Non-U.S. Gov't
       Variation (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).