Document 0400
 DOCN  M9460400
 TI    Modulation of the phenotype and function of bovine afferent lymph cells
       during infection with Trypanosoma congolense.
 DT    9404
 AU    Flynn JN; McKeever DJ; Sileghem M; Naessens J; International Laboratory
       for Research on Animal Diseases (ILRAD),; Nairobi, Kenya.
 SO    Vet Immunol Immunopathol. 1994 Jan;40(1):17-29. Unique Identifier :
       AIDSLINE MED/94174699
 AB    Alterations in the phenotype and function of cells isolated from bovine
       afferent lymph were studied following tsetse-transmitted Trypanosoma
       congolense infection. Little alteration was observed in the output of
       the CD2+ T cells in the lymph, and within this population the CD4:CD8
       ratio remained relatively constant. By contrast, a marked decrease was
       observed in the output of gamma delta T cells over the first 7 days
       following infection. The number of B cells increased between 2 and 6
       days post-infection, and thereafter returned to pre-infection values.
       Little change was observed within the afferent lymph veiled cell
       population. Examination of activation markers on the lymphocyte fraction
       of afferent lymph revealed a decrease in the number of cells expressing
       the Interleukin-2 receptor alpha-chain from Day 5 post-infection. At
       this time the expression of ACT 1, another early activation marker, was
       seen to increase. Afferent lymph cells collected pre-infection and on
       the first 4 days post-infection proliferated in response to stimulation
       with Concanavalin A in vitro. This response to mitogenic stimulation was
       completely abrogated from day five post-infection. However, these cells
       were not capable of suppressing the capacity of normal peripheral blood
       mononuclear cells to respond to mitogenic stimulus in co-culture assays.
       These studies suggest that although a degree of lymphocyte activation
       occurs in the afferent lymph following tsetse-transmitted infection with
       T. congolense, this may be sub-optimal owing to the immunosuppression
       which appears to operate at the level of the skin and the lymph nodes.
 DE    Animal  B-Lymphocytes/*IMMUNOLOGY  Cattle  Cells, Cultured  CD4-CD8
       Ratio  Dendritic Cells  Flow Cytometry/VETERINARY
       Immunophenotyping/VETERINARY  Lymph/CYTOLOGY  Lymphocyte Transformation
       Receptors, Antigen, T-Cell, gamma-delta/IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY  Trypanosoma congolense/*IMMUNOLOGY
       Trypanosomiasis, Bovine/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).