Document 0401
 DOCN  M9460401
 TI    The detection and quantification of feline immunodeficiency provirus in
       peripheral blood mononuclear cells using the polymerase chain reaction.
 DT    9404
 AU    Lawson M; Meers J; Blechynden L; Robinson W; Greene W; Carnegie P;
       School of Biological and Environmental Sciences, Murdoch; University,
       Australia.
 SO    Vet Microbiol. 1993 Dec;38(1-2):11-21. Unique Identifier : AIDSLINE
       MED/94174684
 AB    The polymerase chain reaction method (PCR) was used to detect feline
       immunodeficiency virus proviral DNA in peripheral blood mononuclear
       cells (PBMC) of a group of 8 experimentally infected cats. The
       proportion of PBMC containing provirus was determined from 6 to 32 weeks
       post inoculation (p.i.) by performing PCR on serially diluted samples of
       PBMC. Primers from the p15 and p24 regions of the gag gene were used and
       Southern hybridization using an end-labelled probe was required to
       confirm primer-specific products. Provirus was detected in 5 of 8 cats
       by 6 weeks p.i. in 50000 PBMC, and in all 8 infected cats by 8 weeks
       p.i. Provirus was not detected in PBMC from any of 3 FIV negative cats.
       The proportion of PBMC containing provirus in individual cats ranged
       from 1 in 70 to 1 in 99600 PBMC. There was no significant decline over
       time in the proportion of PBMC containing provirus. Sequencing of a
       segment (287 bases) of the gag region of a West Australian FIV isolate
       (T90) revealed only slight nucleotide divergence from the North American
       Petaluma and PPR isolates and wider divergence from the Japanese TM2
       clone.
 DE    Animal  Base Sequence  Blotting, Southern/VETERINARY  Cats  Cell Line
       Consensus Sequence  DNA Primers/CHEMISTRY  DNA, Viral/ANALYSIS/CHEMISTRY
       Feline Acquired Immunodeficiency Syndrome/*MICROBIOLOGY  Gene Products,
       gag/GENETICS  Genes, gag  Immunodeficiency Virus, Feline/GROWTH &
       DEVELOPMENT/GENETICS/  *ISOLATION & PURIF  Leukocytes,
       Mononuclear/*MICROBIOLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction/VETERINARY  Proviruses/GROWTH & DEVELOPMENT/GENETICS/*ISOLATION
       & PURIF  Random Allocation  RNA, Viral/CHEMISTRY/GENETICS  Sensitivity
       and Specificity  Support, Non-U.S. Gov't  Transcription, Genetic
       Viremia/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).