Document 0438
 DOCN  M9460438
 TI    Development of a flow cytometric assay for the detection and measurement
       of neutralizing antibodies against human immunodeficiency virus.
 DT    9404
 AU    Folghera S; Fiorentini S; Martinelli F; Ravizzola G; Gargiulo F;
       Terlenghi L; De Francesco M; Caruso A; Turano A; Institute of
       Microbiology, University of Brescia, Italy.
 SO    Microbiologica. 1994 Jan;17(1):21-8. Unique Identifier : AIDSLINE
       MED/94173177
 AB    Cells infected with human immunodeficiency virus type 1 (HIV-1) develop
       viral antigens which can be detected by immunofluorescence. We developed
       a flow cytometric immunofluorescence assay (FIFA) which provides a
       quantitative analysis of HIV-1 p24 using a monoclonal antibody (mAb) as
       a specific reagent. The reduction of HIV p24 antigen expression in viral
       infected cells was then used to determine HIV neutralizing antibody
       titers in human sera. Results obtained by FIFA for detecting
       neutralizing antibodies against HIV-1, when compared with results
       obtained by indirect immunofluorescence assay (IFA), showed an overall
       index of agreement of 94.1%. The correlation between the neutralizing
       antibody titers obtained with each method was found to be highly
       significant (ro = 0.8; p < 0.01). The simple methodology and the
       adaptability of this FIFA to various assay conditions make it suitable
       for routine purposes and for assessing the efficacy of vaccination
       strategies.
 DE    Antibodies, Monoclonal  Cell Line  Cell Separation  Comparative Study
       Flow Cytometry/*METHODS  *Fluorescent Antibody Technique  Human  HIV
       Antibodies/*BLOOD  HIV Core Protein p24/*ANALYSIS  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Neutralization Tests
       Reproducibility of Results  Sensitivity and Specificity  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

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