Document 0449
 DOCN  M9460449
 TI    Lentivirus cross-reactive determinants present in the capsid protein of
       equine infectious anaemia virus.
 DT    9404
 AU    Grund CH; Lechman ER; Issel CJ; Montelaro RC; Rushlow KE; Department of
       Molecular Genetics and Biochemistry, School of; Medicine, University of
       Pittsburgh, Pennsylvania 15261.
 SO    J Gen Virol. 1994 Mar;75 ( Pt 3):657-62. Unique Identifier : AIDSLINE
       MED/94172342
 AB    In this study we used immune sera from equine infectious anaemia virus
       (EIAV)-infected horses which uniquely display broad reactivity with
       different lentivirus capsid proteins (CA) to characterize the
       cross-reactive determinants of lentivirus CA proteins. In particular,
       the role of the major homology region (MHR) of lentivirus CA proteins in
       this serological cross-reactivity was evaluated using both equine immune
       serum and murine monoclonal antibodies (MAbs) directed against the MHR
       segment of different lentiviruses. The results of our studies indicate
       that about 80% of sera from long-term experimentally infected ponies or
       naturally infected horses react with human immunodeficiency virus type 1
       CA in Western immunoblot assays. In addition, the cross-reactive
       determinants on the EIAV CA were localized within the immunodominant
       carboxyl terminus of the protein (residues 277 to 367). However, the
       cross-reactive determinants recognized by the equine sera do not appear
       to correlate with linear peptides from the carboxyl terminus of the EIAV
       CA, including the MHR. These results suggest cross-reactivity between
       more distant lentiviruses is associated with non-linear determinants. In
       contrast, MHR-specific MAbs did react with linear peptides by ELISA and
       distinguished the primate lentiviruses from EIAV and feline
       immunodeficiency virus. These data support the concept of a highly
       conserved structural and antigenic organization among the CA proteins of
       lentiviruses from different species.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal  Antigenic
       Determinants/*IMMUNOLOGY  Antigens, Viral/*IMMUNOLOGY
       Capsid/*IMMUNOLOGY  Cross Reactions/IMMUNOLOGY  Equine Infectious
       Anemia/IMMUNOLOGY  Horses  Infectious Anemia Virus, Equine/*IMMUNOLOGY
       Lentivirus/*IMMUNOLOGY  Molecular Sequence Data  Prevalence  Sensitivity
       and Specificity  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).