Document 0469
 DOCN  M9460469
 TI    CD8+ cytolytic T lymphocytes become infected in vitro in the process of
       killing HIV-1-infected target cells.
 DT    9404
 AU    De Maria A; Colombini S; Schnittman SM; Moretta L; Department of
       Infectious Diseases, Ospedale S. Martino, Genova,; Italy.
 SO    Eur J Immunol. 1994 Mar;24(3):531-6. Unique Identifier : AIDSLINE
       MED/94170821
 AB    In the present study the requirements for in vitro infection of
       antigen-specific CD8+ cytotoxic T lymphocytes (CTL) with human
       immunodeficiency virus -1(HIV-1) were investigated. CD3+CD8+CD4- HIV-1
       nef-specific CTL become infected with HIV-1 after short-term co-culture
       with HLA-matched HIV-1-infected CD20+ B lymphoblastoid cells (B-LCL)
       which are specifically killed. Similar results were observed with an
       allospecific CD8+ CTL population. In addition, co-culture experiments
       showed that once infected with HIV-1, these CD8+ CTL could spread the
       infection further to uninfected CD4+ lymphocytes. In contrast, CD8+ CTL
       did not become infected with HIV-1 when co-cultured with HLA-mismatched
       HIV-1-infected B-LCL which are not killed. These observations in vitro
       could have relevance in peripheral lymphoid organs contributing to the
       progressive decrease of HIV-specific CD8+ CTL activity that is
       associated with the progression to AIDS.
 DE    Antigens, CD8/ANALYSIS  Cells, Cultured  Cytotoxicity, Immunologic  DNA,
       Viral/ANALYSIS  Gene Products, nef/IMMUNOLOGY  Human  HIV
       Infections/*MICROBIOLOGY/TRANSMISSION  HIV-1/GROWTH & DEVELOPMENT
       Immunity, Cellular  In Vitro  Lymphoma, B-Cell/MICROBIOLOGY  Support,
       Non-U.S. Gov't  T-Lymphocyte Subsets/*MICROBIOLOGY  T-Lymphocytes,
       Cytotoxic/*MICROBIOLOGY  Tumor Cells, Cultured  T4
       Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

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