Document 0503
 DOCN  M9460503
 TI    Cytokine production by T-cell lines derived from tumor-infiltrating
       lymphocytes from patients with ovarian carcinoma: tumor-specific immune
       responses and inhibition of antigen-independent cytokine production by
       ovarian tumor cells.
 DT    9404
 AU    Kooi S; Freedman RS; Rodriguez-Villanueva J; Platsoucas CD; Department
       of Immunology, University of Texas, M.D. Anderson; Cancer Center,
       Houston 77030.
 SO    Lymphokine Cytokine Res. 1993 Dec;12(6):429-37. Unique Identifier :
       AIDSLINE MED/94169267
 AB    The ability of T-cell lines derived from tumor-infiltrating lymphocytes
       (TIL) from patients with ovarian carcinoma to produce interferon-gamma
       (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and IL-6 in
       response to autologous or allogeneic ovarian carcinoma tumor cells was
       determined. These T-cell lines were derived in low concentrations of
       recombinant IL-2 (rIL-2), 200 IU/ml. Certain of these T-cell lines (3 of
       7) exhibited antigen-independent (spontaneous) production of moderate to
       low concentrations of IFN-gamma (75 to 112 pg/ml), TNF-alpha (62-88
       pg/ml), and IL-6 (38 to 690 pg/ml), in culture medium alone in the
       absence of rIL-2, tumor cells or polyclonal activators. Addition of 20
       IU/ml of rIL-2 to the cultures resulted in a significant increase of the
       antigen-independent production of IFN-gamma (range 75-6480 pg/ml) by all
       seven T-cell lines and TNF-alpha (44-490 pg/ml) by 5 of 7 T-cell lines.
       Certain T-cell lines (4 of 7) exhibited antigen-independent production
       of IL-6 (38-690 pg/ml), which was not affected by the addition of 20
       IU/ml of rIL-2. Certain TIL-derived T-cell lines (2 of 7) produced
       IFN-gamma, TNF-alpha, and IL-6 in response to stimulation with
       autologous ovarian tumor cells, in the presence of 20 IU/ml of rIL-2.
       Irradiated autologous ovarian tumor cells alone or in the presence of
       rIL-2 did not produce any detectable levels of IFN-gamma, TNF-alpha, or
       IL-6 during the 6 day culture.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Antigens, CD/ANALYSIS  Cell Line  Cytokines/ANALYSIS/*BIOSYNTHESIS
       Cytotoxicity, Immunologic  CD4-CD8 Ratio  Enzyme-Linked Immunosorbent
       Assay  Female  Human  Immunophenotyping  Interferon Type II/BIOSYNTHESIS
       Interleukin-6/BIOSYNTHESIS  Lymphocytes,
       Tumor-Infiltrating/IMMUNOLOGY/*METABOLISM  Ovarian Neoplasms/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/IMMUNOLOGY/*METABOLISM  Tumor Cells, Cultured  Tumor
       Necrosis Factor/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).