Document 0519
 DOCN  M9460519
 TI    Role of protein kinase C isozymes in activation of human
       immunodeficiency virus type 1 in chronically infected promonocytic
       cells: evidence against a role of PKC beta 1.
 DT    9404
 AU    Kim CH; Lim SJ; Gollapudi S; Gupta S; Division of Basic and Clinical
       Immunology, University of; California, Irvine 92717.
 SO    Biochem Biophys Res Commun. 1994 Feb 28;199(1):292-7. Unique Identifier
       : AIDSLINE MED/94168589
 AB    Protein kinase C (PKC) plays an important role in activation of human
       immunodeficiency virus type 1 (HIV-1). Because of a molecular and
       biochemical heterogeneity of PKC, we have studied the effects of PKC
       isozymes in HIV-1 activation in a latently infected promonocytic cell
       line, U1, using various PKC isozyme agonists. 12-Deoxyphorbol
       13-phenylacetate (dPP), an agonist of both Ca(++)-dependent and
       Ca(++)-independent isozymes, and thymeleatoxin (TT), an agonist of
       Ca(++)-dependent PKC isozymes, induced HIV-1 production at 10 nM with
       increase in a concentration dependent manner, whereas 12-deoxyphorbol
       13-phenylacetate 20-acetate (dPPA), an PKC beta I isozyme agonist, did
       not induce viral production at 100 nM. We verified that dPPA induced
       translocation of PKC beta isozyme with the isozyme-specific monoclonal
       antibody using flow cytometry. This study demonstrates that activation
       of PKC isozymes leads to an induction of latent HIV-1 in U1 cells
       whereas PKC beta I isozyme may not be important.
 DE    Cell Survival/DRUG EFFECTS  Cells, Cultured  Enzyme Activation  Human
       HIV-1/*GROWTH & DEVELOPMENT  In Vitro  Isoenzymes/ANTAGONISTS &
       INHIB/PHYSIOLOGY  Monocytes/*MICROBIOLOGY  Protein Kinase C/ANTAGONISTS
       & INHIB/*PHYSIOLOGY  Virus Latency  *Virus Replication  JOURNAL ARTICLE

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