Document 0534
 DOCN  M9460534
 TI    Tat and rev differentially affect restricted replication of human
       immunodeficiency virus type 1 in various cells.
 DT    9404
 AU    Duan L; Oakes JW; Ferraro A; Bagasra O; Pomerantz RJ; Dorrance H.
       Hamilton Laboratories, Department of Medicine,; Jefferson Medical
       College, Thomas Jefferson University,; Philadelphia, Pennsylvania 19107.
 SO    Virology. 1994 Mar;199(2):474-8. Unique Identifier : AIDSLINE
       MED/94167884
 AB    Human immunodeficiency virus type 1 (HIV-1) can infect various cell
       lines in culture and be maintained in a chronic state of restricted
       replication. These states of proviral latency are characterized by a
       predominance of spliced compared to unspliced viral RNA species. The
       proximate molecular mechanisms leading to restricted HIV-1 replication
       may differ in various cell lines. Importantly, recent studies have
       demonstrated that the site of integration is the critical parameter
       leading to proviral latency in ACH-2 cells. Utilizing murine retroviral
       shuttle vectors, the HIV-1 Tat protein was demonstrated to dramatically
       increase HIV-1 expression in the restrictively infected U1 monocytic
       cell line but not in the ACH-2 T-lymphocytic line. The HIV-1 Rev protein
       only modestly increased viral expression in both of these cell types.
       Thus, these data support the hypothesis that the mechanisms which
       initiate and/or maintain restricted HIV-1 expression may differ in
       various cell types in cell culture, and possibly in vivo.
 DE    Base Sequence  Cell Line/*MICROBIOLOGY  Gene Products, rev/*PHYSIOLOGY
       Gene Products, tat/GENETICS/*PHYSIOLOGY  HIV-1/*PHYSIOLOGY  Molecular
       Sequence Data  Recombinant Proteins  RNA, Viral/ANALYSIS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virus
       Replication/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).