Document 0535
 DOCN  M9460535
 TI    HIV-2 viral protein X association with the GAG p27 capsid protein.
 DT    9404
 AU    Horton R; Spearman P; Ratner L; Department of Medicine, Washington
       University School of Medicine,; St. Louis, Missouri 63110.
 SO    Virology. 1994 Mar;199(2):453-7. Unique Identifier : AIDSLINE
       MED/94167880
 AB    VPX is a 16 kDa accessory protein expressed in cells infected with HIV-2
       and most SIV strains and is packaged into virus particles. In order to
       define the requirements for incorporation of VPX into virions, VPX and
       HIV-2 GAG-POL were expressed independently from a vaccinia virus-based
       transient expression system. Under these conditions, VPX was exported
       from transfected cells only when coexpressed with the HIV-2 GAG-POL
       plasmid. A 27 kDa protein coprecipitating with VPX was found to have an
       identical electrophoretic mobility as the GAG capsid protein, and
       reacted with an anti-GAG antiserum. Coexpression of VPX and GAG-POL
       resulted in virus-like particles containing both proteins, as determined
       by sucrose gradient analyses. Expression of VPX and HIV-2 GAG without
       POL gave similar results. VPX association with HIV-2 GAG p27 capsid
       protein was specific, since no association was found with the HIV-1 GAG
       p25/p24 capsid protein.
 DE    Animal  Capsid/*METABOLISM  Cell Line  Cercopithecus aethiops  Fusion
       Proteins, gag-pol/METABOLISM  Gene Products, gag/*METABOLISM
       HIV-2/*METABOLISM  Retroviridae Proteins/*METABOLISM  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).