Document 0537
 DOCN  M9460537
 TI    Complementation of human immunodeficiency virus (HIV-1) gag particle
       formation.
 DT    9404
 AU    Zhoa Y; Jones IM; Hockley DJ; Nermut MV; Roy P; Department of
       Biochemistry, University of Oxford.
 SO    Virology. 1994 Mar;199(2):403-8. Unique Identifier : AIDSLINE
       MED/94167874
 AB    The human immunodeficiency virus gag precursor protein Pr55Gag exhibits
       the ability of particle assembly when expressed using recombinant
       baculoviruses. In order to delineate the sequences required for particle
       formation, two mutants of Gag (D1 and D2) were constructed in which 10
       amino acids within the CA domain were deleted. Both mutants yielded
       stable high levels of Gag antigen following expression in Spodoptera
       frugiperda insect cells. Electron microscopy of sections through
       infected cells revealed that neither mutant was able to assemble
       particles although targeting of the protein to the plasma membrane still
       occurred. The Gag antigen that accumulated beneath the plasma membrane
       exhibited distinctive morphologies when compared to each other and to
       parental (Pr46Gag) particles. Particle assembly was rescued when S.
       frugiperda cells were coinfected with both AcD1 and AcD2 viruses, or
       with AcD1 and a carboxyl-terminal deletion of Gag (Pr41.5) which was
       previously shown not to form particles (J.B.M., D.J. Hockley, M.V.
       Nermot, and I.M. Jones, 1992, J. Gen. Virol. 73, 3079-3086). The genetic
       complementation of Gag-driven assembly is discussed.
 DE    Amino Acid Sequence  Animal  Antigens, Viral/*BIOSYNTHESIS
       Baculoviridae  Capsid/GENETICS/*METABOLISM  Gene Expression/PHYSIOLOGY
       Gene Products, gag/GENETICS/*METABOLISM  HIV-1/GENETICS/*METABOLISM
       Molecular Sequence Data  Moths  Recombinant Proteins/BIOSYNTHESIS
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).