Document 0540
 DOCN  M9460540
 TI    trans-activation of the HIV promoter by a cDNA and its genomic clones of
       human herpesvirus-6.
 DT    9404
 AU    Zhou Y; Chang CK; Qian G; Chandran B; Wood C; Department of Neurology
       and Microbiology, University of Miami; School of Medicine, Florida
       33101.
 SO    Virology. 1994 Mar;199(2):311-22. Unique Identifier : AIDSLINE
       MED/94167865
 AB    Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro,
       it can productively infect human CD4+ T cells as HIV-1. Co-infection of
       T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1
       promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA
       clone, pCD41, encoding for a 41-kDa nuclear protein was identified and
       characterized previously (Chang and Balachandran, J. Virol. 65,
       2884-2894 and 7085, 1991). Sequence analyses show that this protein has
       significant homology with the human cytomegalovirus UL44 gene coding for
       the ICP36 family of early-late-class phosphoprotein. Using this cDNA as
       the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was
       cloned and designated as pGD41. When cotransfected with the HIV LTR CAT
       into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV
       LTR. Sequence analyses of pCD41 indicate that there are two potential
       open reading frames (ORFs), A and B, which are homologous to the ORFs
       found in the genomic clone pGD41. Deletion constructs of the pCD41 clone
       demonstrated that ORF-A was critical for the HIV LTR activation.
       Deletion analyses of the pCD41 ORF-A and the use of promoter constructs
       further mapped an internal functional promoter within the pCD41 sequence
       that can direct the synthesis of the trans-activating protein. By using
       HIV LTR deletion mutants, the NF-kappa B binding sites were found to be
       critical for response to the pCD41 trans-activation.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Cercopithecus
       aethiops  Cloning, Molecular  DNA, Complementary/GENETICS/*PHYSIOLOGY
       Gene Expression Regulation, Viral/PHYSIOLOGY  Herpesvirus 6,
       Human/GENETICS/*PHYSIOLOGY  Human  HIV Long Terminal Repeat/*PHYSIOLOGY
       HIV-1  Molecular Sequence Data  NF-kappa B/PHYSIOLOGY  Open Reading
       Frames/PHYSIOLOGY  Phosphoproteins/GENETICS  Promoter Regions
       (Genetics)/GENETICS/PHYSIOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes  Trans-Activation
       (Genetics)/GENETICS/*PHYSIOLOGY  Viral Proteins/*GENETICS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).