Document 0553
 DOCN  M9460553
 TI    Detection of Epstein-Barr genome in the lymph nodes of Hodgkin's
       disease.
 DT    9404
 AU    Bellas C; Mampaso F; Fraile G; Molina A; Bricio T; Cuesta C; Department
       of Pathology, Hospital Ramon y Cajal, Madrid, Spain.
 SO    Postgrad Med J. 1993 Dec;69(818):916-9. Unique Identifier : AIDSLINE
       MED/94167327
 AB    We have used the enzymatic in situ hybridization method to investigate
       the presence of Epstein-Barr virus (EBV) genome in lymph node tissues
       from patients with Hodgkin's disease. Also, 11 patients with persistent
       human immuno-deficiency virus-associated generalized lymphadenopathy as
       well as seven autopsy cases with no Hodgkin's disease, formed part of
       these studies. EBV DNA-positive reaction was demonstrated in
       Reed-Sternberg cells and variants in seven of 16 cases and in the small
       accompanying lymphocyte cell population in 14 of the 16 cases. It was
       also found in eight of the 11 cases with persistent generalized
       lymphadenopathy but in none of the lymph nodes from negative selective
       autopsy cases. Our results indicate that the colorimetric in situ
       hybridization technique is useful in EBV nucleic acid detection and
       cell-type localization in Hodgkin's disease. Additionally, the detection
       of EBV genome, not only in the diagnostic cells but in the small
       lymphocyte cell components, could provide new insights into the
       potential role of this agent in the pathogenesis of Hodgkin's disease.
 DE    Adolescence  Adult  Aged  AIDS-Related Complex/MICROBIOLOGY  Child
       Comparative Study  DNA, Viral/*ANALYSIS  Female  Herpesvirus 4,
       Human/*GENETICS  Hodgkin's Disease/*MICROBIOLOGY  Human  In Situ
       Hybridization  Lymph Nodes/*CHEMISTRY  Male  Middle Age  Reed-Sternberg
       Cells/CHEMISTRY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

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