Document 0555
 DOCN  M9460555
 TI    Dimerization of human immunodeficiency virus type 1 RNA involves
       sequences located upstream of the splice donor site.
 DT    9404
 AU    Marquet R; Paillart JC; Skripkin E; Ehresmann C; Ehresmann B; Unite
       Propre de Recherche 9002, Centre National de la Recherche; Scientifique,
       Strasbourg, France.
 SO    Nucleic Acids Res. 1994 Jan 25;22(2):145-51. Unique Identifier :
       AIDSLINE MED/94167239
 AB    The retroviral genome consists of two homologous RNA molecules
       associated close to their 5' ends. We studied the spontaneous
       dimerization of four HIV-1 RNA fragments (RNAs 1-707, 1-615, 311-612,
       and 311-415) containing the previously defined dimerization domain, and
       a RNA fragment (RNA 1-311) corresponding to the upstream sequences.
       Significant dimerization of all RNAs is observed on agarose gels when
       magnesium is included in the electrophoresis buffer. In contrast to
       dimerization of RNAs 311-612 and 311-415, dimerization of RNAs 1-707,
       1-615 and 1-311 strongly depends on the size of the monovalent cation
       present in the incubation buffer. Also, dimerization of RNAs 1-707,
       1-615, and 1-311 is 10 times faster than that of RNAs 311-612 and
       311-415. The dimers formed by the latter RNAs are substantially more
       stable than that of RNA 1-615, while RNA 1-311 dimer is 5-7 degrees C
       less stable than RNA 1-615 dimer. These results indicate that
       dimerization of HIV-1 genomic RNA involves elements located upstream of
       the splice donor site (position 305), i.e. outside of the previously
       defined dimerization domain.
 DE    Base Sequence  Cations  HIV-1/*GENETICS  Kinetics  Macromolecular
       Systems  Magnesium  Models, Chemical  Models, Genetic  Molecular
       Sequence Data  RNA Splicing  RNA, Viral/*CHEMISTRY  Support, Non-U.S.
       Gov't  Thermodynamics  JOURNAL ARTICLE

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