Document 0556
 DOCN  M9460556
 TI    Premature strand transfer by the HIV-1 reverse transcriptase during
       strong-stop DNA synthesis.
 DT    9404
 AU    Klaver B; Berkhout B; University of Amsterdam, Department of Virology,
       The Netherlands.
 SO    Nucleic Acids Res. 1994 Jan 25;22(2):137-44. Unique Identifier :
       AIDSLINE MED/94167238
 AB    Reverse transcription of retroviral genomes starts near the 5' end of
       the viral RNA by use of an associated tRNA primer. According to the
       current model of reverse transcription, the initial cDNA product, termed
       minus-strand strong-stop DNA, 'jumps' to a repeated sequence (R region)
       at the 3' end of the RNA template. The human retroviruses have
       relatively long R regions (97-247 nucleotides) when compared to murine
       and avian viruses (16-68 nucleotides). This suggests that the full
       complement of the R region is not required for strand transfer and that
       partial cDNA copies of the 5' R can prematurely jump to the 3' R. To
       test this hypothesis, we generated mutants of the human immunodeficiency
       virus with R region changes and analyzed whether 5' or 3' R sequences
       were inherited by the progeny. We found that in most cases, 5' R-encoded
       sequences are dominant, which is consistent with the model of reverse
       transcription. Using a selection protocol, however, we were also able to
       identify progeny viruses with R sequences derived from the original 3' R
       element. These results suggest that partial strong stop cDNAs can be
       transferred with R region homologies much shorter than 97 nucleotides.
 DE    Base Sequence  Cell Line  DNA, Viral/*BIOSYNTHESIS  HIV Long Terminal
       Repeat  HIV-1/*GENETICS  Molecular Sequence Data  Mutation
       Recombination, Genetic  Reverse Transcriptase/*METABOLISM  RNA,
       Viral/*GENETICS  Support, Non-U.S. Gov't  Templates  Transcription,
       Genetic/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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