Document 0558
 DOCN  M9460558
 TI    [Contribution of immunoblot to the diagnosis and monitoring of
       toxoplasmosis in acquired immunodeficiency syndrome]
 DT    9404
 AU    Franck J; Mary C; Jarroux E; Dumon H; Quilici M; Laboratoire de
       Parasitologie, Faculte de Medecine, Marseille,; France.
 SO    Pathol Biol (Paris). 1993 Nov;41(9):865-72. Unique Identifier : AIDSLINE
       MED/94167152
 AB    This study evaluates the potential value of immunoblot for serological
       survey of toxoplasmosis infection in HIV infected patients. This
       technique was applied to the detection of IgG, IgM, IgA and IgE
       antibodies to Toxoplasma gondii in serum and cerebrospinal fluid. We
       compared the patterns obtained from sera of immunocompetent and HIV+
       subjects in different situations: absence of toxoplasmosis,
       primo-infection, chronic infection and reactivation of toxoplasmosis.
       IgA antibodies were demonstrated in sera of all HIV+ patients with
       chronic infection: so, these antibodies have any diagnosis value in this
       group. IgE were only detected by immunoblot when clinical symptoms of
       toxoplasmosis occurred in cases of HIV+ patients with reactivation of
       the parasitosis; previously, these subjects presented a serological rise
       of IgG specific antibodies. In ten cases of neurotoxoplasmosis
       immunoblot allowed detection of IgA and IgE antibodies in serum and CSF
       and demonstrated their intrathecal synthesis in three cases. In
       conclusion immunoblot is a powerful technique for detection of IgA and
       IgE to Toxoplasma gondii. Demonstration of IgE antibodies seem to be a
       good criterion to evaluate the evolutivity of the parasitosis in HIV
       patients.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*DIAGNOSIS/  IMMUNOLOGY
       AIDS-Related Opportunistic Infections/COMPLICATIONS/*DIAGNOSIS/
       IMMUNOLOGY  Blotting, Western/*METHODS  Chronic Disease  English
       Abstract  Human  IgA/ANALYSIS  IgE/ANALYSIS  IgG/ANALYSIS  IgM/ANALYSIS
       Monitoring, Physiologic  Toxoplasmosis,
       Cerebral/COMPLICATIONS/*DIAGNOSIS/IMMUNOLOGY  Toxoplasmosis,
       Ocular/COMPLICATIONS/*DIAGNOSIS/IMMUNOLOGY  JOURNAL ARTICLE

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