Document 0561
 DOCN  M9460561
 TI    Direct interaction of human TFIID with the HIV-1 transactivator tat.
 DT    9404
 AU    Kashanchi F; Piras G; Radonovich MF; Duvall JF; Fattaey A; Chiang CM;
       Roeder RG; Brady JN; Laboratory of Molecular Virology, National Cancer
       Institute,; National Institutes of Health, Bethesda, Maryland 20892.
 SO    Nature. 1994 Jan 20;367(6460):295-9. Unique Identifier : AIDSLINE
       MED/94166886
 AB    The tat gene of the human immunodeficiency virus (HIV) plays a central
       role in the activation and life cycle of HIV. The tat protein (Tat)
       specifically transactivates HIV transcription in vivo and in vitro,
       exerting its effects at the level of transcriptional initiation and
       elongation. Here we report that Tat binds directly to the basal
       transcription factor TFIID. The transcriptional activity of HeLa
       extracts was depleted after chromatography on a Tat affinity column,
       which specifically retained the polymerase II-specific factor TFIID.
       Direct interaction of Tat with holo-TFIID, composed of TATA-binding
       protein (TBP) and associated factors (TAFs), was observed. Tat binds,
       through amino acids 36-50, directly to the TBP subunit of TFIID. Our
       results suggest that Tat may transduce upstream or downstream regulatory
       signals by direct interaction with the basal transcription factor TFIID.
 DE    Base Sequence  DNA-Binding Proteins/METABOLISM  DNA, Viral  Gene
       Products, tat/GENETICS/*METABOLISM  Glutathione Transferases/METABOLISM
       Hela Cells  Human  HIV-1/*METABOLISM  Molecular Sequence Data  Mutation
       Protein Binding  Recombinant Fusion Proteins/METABOLISM  Signal
       Transduction  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Transcription Factors/*METABOLISM  Transcription, Genetic  JOURNAL
       ARTICLE

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