Document 0571
 DOCN  M9460571
 TI    [Two-color flow cytometric analysis of lymphocytic subsets in renal cell
       carcinoma]
 DT    9404
 AU    Nagase Y; Moriyama N; Kurimoto S; Hosaka Y; Tajima A; Higashihara E; Aso
       Y; Department of Urology, Faculty of Medicine, University of Tokyo.
 SO    Nippon Hinyokika Gakkai Zasshi. 1994 Feb;85(2):281-8. Unique Identifier
       : AIDSLINE MED/94166315
 AB    The subsets of tumor infiltrating lymphocytes (TILs) in 17 patients with
       renal cell carcinoma were studied by two-color flow cytometry and
       immunohistochemistry. In grade 1 tumors, CD4-positive cells were
       significantly increased compared to grade 2 and 3 tumors (35 +/- 7% vs
       25 +/- 10%) (p < 0.05). In 10 of 14 patients, CD8-positive cells were
       predominant over CD4-positive cells, resulting in less than one in
       CD4/CD8-ratio. Regarding CD4 cells in all 17 cases, the helper T-cells
       (CD4-positive and CD45RA-negative) were statistically predominant over
       the inducer T-cells (CD4-positive and CD45RA-positive) (p < 0.01). When
       CD8 cells were investigated in 16 out of the 17 cases, the cytotoxic
       T-cells (CD8-positive and CD11b-negative) were found also significantly
       predominant over the suppressor T-cells (CD8-positive and
       CD11b-positive) (p < 0.01). Immunohistochemically, the infiltrating
       pattern of TILs in the 17 patients was classified into cluster (N = 4),
       separate (N = 6), and mixed types (N = 7). The tumor in the cluster and
       mixed type had a tendency to be dominant in low grade tumors, and had
       the prominent CD8-positive cells (more than 90% were cytotoxic T-cells)
       over CD4-positive cells. However, no statistical significance was noted
       in this finding. The advanced tumor tended to show the single type of
       TIL infiltration. These results suggest that cytotoxic T-cells consisted
       of most of TILs in cluster and mixed types play an immunoreactive role
       against renal cell carcinoma.
 DE    Adult  Aged  Aged, 80 and over  Carcinoma, Renal Cell/*IMMUNOLOGY
       CD4-CD8 Ratio  English Abstract  Female  Flow Cytometry/METHODS  Human
       Immunohistochemistry  Kidney Neoplasms/*IMMUNOLOGY  *Lymphocyte Subsets
       *Lymphocytes, Tumor-Infiltrating  Male  Middle Age  JOURNAL ARTICLE

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