Document 0580
 DOCN  M9460580
 TI    Vitamin E is an important factor in T cell differentiation in thymus of
       F344 rats.
 DT    9404
 AU    Moriguchi S; Miwa H; Okamura M; Maekawa K; Kishino Y; Maeda K;
       Department of Nutrition, School of Medicine, University of; Tokushima,
       Japan.
 SO    J Nutr Sci Vitaminol (Tokyo). 1993 Oct;39(5):451-63. Unique Identifier :
       AIDSLINE MED/94165838
 AB    The effect of vitamin E (dl-alpha-tocopheryl acetate) on T cell
       differentiation in thymus of F344 rats was examined in this study. The
       rats were divided into three groups: vitamin E-free, regular and high
       vitamin E groups and fed a diet containing various levels of vitamin E
       (0, 50, and 585 mg/kg diet) for 7 weeks. The number of thymocytes was
       significantly lower in the vitamin E-free group relative to the regular
       group. Although the proportions of both CD4+CD8- and CD4-CD8+ T cells in
       thymocytes were significantly greater in the high vitamin E group, the
       proportion of CD4+CD8- T cells inversely decreased in vitamin E-free
       group compared to that of the regular group. The ratio of
       CD4+CD8-/CD4-CD8+ T cells increased in the high vitamin E group (p <
       0.01) and significantly decreased in the vitamin E-free group (p <
       0.001) compared to that of the regular group. Although the marked
       changes of T cell subsets were not seen in peripheral blood lymphocytes
       (PBL), the ratio of CD4+CD8-/CD4-CD8+ T cells was significantly lower in
       the vitamin E-free group and significantly greater in the high vitamin E
       group compared to that of the regular group. Production of interleukin
       (IL) 2 by thymocytes following the stimulation with Con A for 48 h
       increased about threefold in the high vitamin E group compared to the
       regular group. Conversely, thymocytes from rats fed the vitamin E-free
       diet showed a significant decrease of IL2 production compared to that of
       the regular group. Prostaglandin E2 (PGE2) production from thymocytes
       was significantly lower in the high vitamin E group compared to that of
       the regular group, whereas thymocytes of rats fed the vitamin E-free
       diet showed a significant increase of PGE2 production compared to that
       of rats fed the regular diet. Furthermore, in vitro addition of
       indomethacin provided a restoration of IL2 production from thymocytes of
       rats fed the vitamin E-free diet to the level of rats fed the regular
       diet. These results suggest that vitamin E plays an important role in T
       cell differentiation in thymus, which may be related to the action of
       vitamin E as antioxidant.
 DE    Animal  Antioxidants/PHARMACOLOGY  Cell Differentiation/DRUG EFFECTS
       Cell Division/DRUG EFFECTS  Concanavalin A/PHARMACOLOGY  CD4-CD8
       Ratio/*DRUG EFFECTS  Diet  Dinoprostone/BIOSYNTHESIS  Flow Cytometry
       Indomethacin/PHARMACOLOGY  Interleukin-2/BIOSYNTHESIS  Lymphocytes/DRUG
       EFFECTS  Male  Phytohemagglutinins/PHARMACOLOGY  Rats  Rats, Inbred F344
       T-Lymphocytes/*DRUG EFFECTS  Thymus Gland/*DRUG EFFECTS/IMMUNOLOGY
       Vitamin E/ANALYSIS/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).