Document 0590
 DOCN  M9460590
 TI    Morphine amplifies HIV-1 expression in chronically infected promonocytes
       cocultured with human brain cells.
 DT    9404
 AU    Peterson PK; Gekker G; Hu S; Anderson WR; Kravitz F; Portoghese PS;
       Balfour HH Jr; Chao CC; Neuroimmunobiology and Host Defense Laboratory,
       Minneapolis; Medical Research Foundation, MN 55404.
 SO    J Neuroimmunol. 1994 Mar;50(2):167-75. Unique Identifier : AIDSLINE
       MED/94165200
 AB    Previous studies have shown that morphine promotes the replication of
       human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear
       cell cocultures. In the present study, we tested the hypothesis that
       morphine would amplify HIV-1 expression in the chronically infected
       promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated
       human fetal brain cells. Marked upregulation of HIV-1 expression was
       observed in these cocultures (quantified by measurement of HIV-1 p24
       antigen levels in supernatants), and treatment of brain cells with
       morphine resulted in a bell-shaped dose-dependent enhancement of viral
       expression. The mechanism of morphine's amplifying effect appears to be
       opioid receptor-mediated and to involve enhanced production of tumor
       necrosis factor-alpha by microglial cells.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY/PATHOLOGY
       Brain/*MICROBIOLOGY/PATHOLOGY  Cells, Cultured  Cytokines/PHYSIOLOGY
       Cytological Techniques  Human  HIV-1/*DRUG EFFECTS/PHYSIOLOGY
       Microglia/METABOLISM  Monocytes/*MICROBIOLOGY  Morphine/*PHARMACOLOGY
       Receptors, Opioid/PHYSIOLOGY  Stem Cells/*MICROBIOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Tumor Necrosis
       Factor/METABOLISM  Virus Replication/*DRUG EFFECTS  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).