Document 0604
 DOCN  M9460604
 TI    Involvement of HIV nef protein in abnormal hematopoiesis in AIDS: in
       vitro study on bone marrow progenitor cells.
 DT    9404
 AU    Calenda V; Graber P; Delamarter JF; Chermann JC; INSERM Unit 322,
       Research Unit on Retroviruses and Associated; Diseases, Marseille,
       France.
 SO    Eur J Haematol. 1994 Feb;52(2):103-7. Unique Identifier : AIDSLINE
       MED/94164276
 AB    In previous studies (1-4), we described disrupted hematopoiesis in
       long-term human bone marrow cultures infected with HIV. Conditioned
       medium (CM) from HIV-1 non-productively infected liquid cultures
       inhibits the proliferation of granulo-monocytic progenitor cells
       (CFU-GM) in clonogenic assays. A growth-inhibitory soluble factor was
       therefore suspected and we report here that this factor has been
       identified as the nef gene product. These nef-containing supernatants
       are specifically neutralized by anti-nef antibodies and recombinant nef
       has the same growth inhibitory properties. We used a nef-deficient virus
       to confirm that nef protein is responsible for the in vitro growth
       inhibition of CFU-GM.
 DE    Acquired Immunodeficiency Syndrome/*BLOOD  Bone
       Marrow/CYTOLOGY/*MICROBIOLOGY  Cell Division  Cells, Cultured  Gene
       Products, nef/*PHYSIOLOGY  Genes, nef  *Hematopoiesis  Hematopoietic
       Stem Cells/*MICROBIOLOGY  Human  In Vitro  Recombinant Proteins
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).