Document 0607
 DOCN  M9460607
 TI    CD4+ class I-restricted T cells specific for HIV gp160 315-329.
 DT    9404
 AU    Moore RL; Fox BS; Department of Microbiology and Immunology, University
       of Maryland; at Baltimore 21201.
 SO    Cell Immunol. 1994 Mar;154(1):43-53. Unique Identifier : AIDSLINE
       MED/94163715
 AB    Mature, circulating, alpha beta T cells express either CD4 or CD8. In
       the majority of cases, CD4+ T cells recognize Ag in association with
       class II MHC molecules, while CD8+ T cells recognize Ag in association
       with class I MHC molecules. In this report we describe CD4+ class
       I-restricted T cell hybridomas and normal clones specific for the
       peptide 315-329 of HIV gp160 strain IIIb in association with H-2Dd. Two
       models were formulated to explain how CD4+ class I-restricted T cells
       could arise. First, they could represent aberrant selection of CD4+
       cells on class I MHC molecules in the thymus. Alternatively, they could
       have been selected normally on class II; in this case the cells would
       display cross-reactive recognition of 315-329 in association with H-2Dd
       and an unknown Ag in association with class II. To distinguish these
       models, a second specificity was determined for the T cell clones. The
       normal clones recognized the class I molecule H-2Kk as alloantigen and
       thus were presumably positively selected in the thymus on class I MHC.
       CD4 was shown to be functional in these cells in that anti-CD4 mAb
       inhibited their proliferation: however, both Ag- and Con A-induced
       responses were inhibited, suggesting that a negative signal was
       delivered by the anti-CD4 mAb.
 DE    Animal  Antigens, CD4/METABOLISM  Clone Cells/IMMUNOLOGY  Gene Products,
       env/*IMMUNOLOGY  H-2 Antigens  *Histocompatibility Antigens Class I
       Histocompatibility Antigens Class II  Hybridomas/IMMUNOLOGY  Isoantigens
       Lymphocyte Transformation  Mice  Mice, Inbred A  Mice, Inbred BALB C
       Mice, Inbred C57BL  Mice, Inbred DBA  Peptide Fragments/*IMMUNOLOGY
       Protein Precursors/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  T4
       Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).