Document 0608
 DOCN  M9460608
 TI    Effect of cytokines on HIV replication in CD4+ lymphocytes: lack of
       identity with the CD8+ cell antiviral factor.
 DT    9404
 AU    Mackewicz CE; Ortega H; Levy JA; Cancer Research Institute, University
       of California, School of; Medicine, San Francisco 94143-0128.
 SO    Cell Immunol. 1994 Feb;153(2):329-43. Unique Identifier : AIDSLINE
       MED/94163683
 AB    CD8+ cells from HIV-infected individuals inhibit HIV replication in
       cultured CD4+ cells by a nonlytic, non-MHC-restricted mechanism. The
       activity appears to be mediated in part by a soluble antiviral factor
       (CAF) secreted by the CD8+ cells. In an attempt to identify this factor
       a large panel of recombinant cytokines was examined for their effect on
       HIV replication in CD4+ cells. In addition to interferon-alpha and
       -beta, TNF alpha, TGF beta, and IL-8 reduced virus replication in a
       dose-dependent fashion. In some cases, the effect of the cytokine was
       also dependent on the HIV infection assay used to measure it. Antibodies
       against the inhibitory cytokines, as well as antibodies against TNF
       beta, IFN-alpha, IFN-beta, IL-4, and IL-6 did not inactivate the
       antiviral effect of CAF. The data suggest that CAF does not have
       identity with known antiviral cytokines and therefore CAF may be a novel
       antiviral factor.
 DE    Antigens, CD8  Antiviral Agents/PHARMACOLOGY  Cytokines/ANTAGONISTS &
       INHIB/*PHARMACOLOGY  Human  HIV-1/*DRUG EFFECTS/IMMUNOLOGY/PHYSIOLOGY
       In Vitro  Support, U.S. Gov't, P.H.S.  T-Lymphocyte Subsets/IMMUNOLOGY
       Transforming Growth Factor beta/PHARMACOLOGY  Tumor Necrosis
       Factor/PHARMACOLOGY  T4 Lymphocytes/*IMMUNOLOGY/*MICROBIOLOGY  Virus
       Replication/*DRUG EFFECTS/*IMMUNOLOGY  2',5'-Oligoadenylate
       Synthetase/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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