Document 0634
 DOCN  M9460634
 TI    An RNA-binding peptide from bovine immunodeficiency virus Tat protein
       recognizes an unusual RNA structure.
 DT    9404
 AU    Chen L; Frankel AD; Department of Biochemistry and Biophysics,
       University of; California, San Francisco 94141.
 SO    Biochemistry. 1994 Mar 8;33(9):2708-15. Unique Identifier : AIDSLINE
       MED/94162291
 AB    The human immunodeficiency virus (HIV) Tat protein binds specifically to
       an RNA hairpin, TAR, located at the 5' end of its mRNA. Tat uses a
       single arginine residue within a short region of basic amino acids to
       recognize a bulge region in TAR. Here we show that a 17 amino acid
       arginine-rich peptide from the bovine immunodeficiency virus (BIV) Tat
       protein also binds to an RNA hairpin at the 5' end of its mRNA (BIV
       TAR), but recognizes different structural features of the RNA.
       Mutagenesis, RNase mapping, and chemical interference experiments
       indicate that bulge and stem regions of BIV TAR are recognized
       simultaneously by the BIV peptide and that the RNA adopts an unusual
       structure. BIV Tat binds to its TAR site with high affinity and
       specificity and, unlike HIV Tat, does not appear to use cellular
       proteins to stabilize RNA binding in vivo. Thus, two related viral
       activators have evolved rather distinct ways to recognize their RNA
       targets.
 DE    Amino Acid Sequence  Arginine/CHEMISTRY  Base Sequence  Gene Products,
       tat/*CHEMISTRY/METABOLISM  Hydrogen Bonding  Immunodeficiency Virus,
       Bovine/*METABOLISM  Molecular Sequence Data  Nucleic Acid Conformation
       Peptides/CHEMISTRY  RNA-Binding Proteins/*CHEMISTRY/METABOLISM  RNA,
       Viral/*METABOLISM/ULTRASTRUCTURE  Sequence Alignment  Sequence Homology,
       Amino Acid  Structure-Activity Relationship  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).