Document 0639
 DOCN  M9460639
 TI    Size exclusion removal of model mammalian viruses using a unique
       membrane system, Part II: Module qualification and process simulation.
 DT    9404
 AU    DiLeo AJ; Vacante DA; Deane EF; Millipore Corporation, Bedford, MA
       01730.
 SO    Biologicals. 1993 Sep;21(3):287-96. Unique Identifier : AIDSLINE
       MED/94161955
 AB    The performance of a fabricated device may be influenced by the
       characteristics of the fluid management within the device and
       reproducibility with which the device is manufactured. The performance
       of the Viresolve/70 membrane is not diminished when incorporated into a
       fabricated module. The Viresolve/70 fabricated modules are shown to
       reproducibly retain viruses via a sieving mechanism, independent of
       virus type or character, in excellent agreement with the base membrane
       retention coefficients reported previously. The retention coefficients
       measured for the Viresolve/70 modules are shown to increase with
       increased recirculation flow rate within the module. Mammalian virus
       spiked protein solutions processed through the Viresolve/70 system show
       that mammalian viruses can be removed from solution in accordance with
       the apparent membrane retention coefficients. The retained virus is
       recoverable on the upstream side of the membrane. Process clearance
       factors for murine leukemia virus is in excess of 6.7 LRV and that of
       human immunodeficiency virus I is 8.5 LRV.
 DE    Animal  Cells, Cultured  Evaluation Studies  Filtration/*METHODS
       HIV-1/ISOLATION & PURIF  Mammals/MICROBIOLOGY  *Membranes, Artificial
       Polioviruses/ISOLATION & PURIF  Polyomavirus macacae/ISOLATION & PURIF
       Rauscher Virus/ISOLATION & PURIF  Reoviridae/ISOLATION & PURIF
       Reproducibility of Results  Sindbis Virus/ISOLATION & PURIF
       Viruses/*ISOLATION & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).