Document 0641
 DOCN  M9460641
 TI    Insufficient inactivation of HIV-1 in human cryo poor plasma by
       beta-propiolactone: results from a highly accurate virus detection
       method.
 DT    9404
 AU    Norley SG; Lower J; Kurth R; Paul-Ehrlich-Institut, Langen, Germany.
 SO    Biologicals. 1993 Sep;21(3):251-8. Unique Identifier : AIDSLINE
       MED/94161951
 AB    Human cryo poor plasma was spiked with high titre HIV-1 and subjected to
       a beta-propiolactone (beta-PL) inactivation procedure. The residual
       titre of HIV-1 was measured using a sensitive and accurate titration
       technique based upon the killing of MT-4 cells by HIV and the subsequent
       inability to incorporate 3H-thymidine. It was found that under these
       conditions a reduction of only 1 log virus titre was achieved after one
       hour exposure to beta-PL and of 1.8 logs after 5 h. The presence of
       viable HIV was confirmed using immunohistochemical techniques, by
       transfer of live virus to fresh cells, and by production of reverse
       transcriptase. These results have obvious implications for products
       where human plasma is treated with beta-PL as the only virus
       inactivation step.
 DE    Blood/*MICROBIOLOGY  Blood Preservation  Cell Line  Cryopreservation
       Human  HIV-1/*DRUG EFFECTS/ISOLATION & PURIF  Immunoenzyme Techniques
       Propiolactone/*PHARMACOLOGY  Thymidine/METABOLISM  Titrimetry  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).