Document 0686
 DOCN  M9460686
 TI    Search for an HTLV-I-like retrovirus in patients with MS by enzymatic
       DNA amplification.
 DT    9404
 AU    Lisby G; Department of Clinical Microbiology, Herlev Hospital, Denmark.
 SO    Acta Neurol Scand. 1993 Dec;88(6):385-7. Unique Identifier : AIDSLINE
       MED/94160692
 AB    A retroviral etiology to MS has been sought for some time, so far
       reports of retroviral presence have not been confirmed by other groups.
       DNA was isolated from peripheral blood mononuclear cells from 67
       patients with MS and brain capillaries from six patients with MS.
       Enzymatic amplification by the polymerase chain reaction was conducted
       with ten primer sets homologous to highly conserved HTLV-I/HTLV-II
       genetic sequences at stringent and non-stringent annealing conditions.
       No HTLV I/II related DNA fragments were seen judging from hybridization
       to an HTLV-I probe, even at relaxed conditions. The present study does
       not support a role for a HTLV-I-like virus in MS.
 DE    DNA/*BLOOD  DNA Primers  Female  *Gene Amplification  Genome, Viral
       Human  HTLV Antibodies/IMMUNOLOGY  Male  Multiple
       Sclerosis/*DIAGNOSIS/ENZYMOLOGY  Oligonucleotides  *Polymerase Chain
       Reaction  Retroviridae/*ISOLATION & PURIF  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).