Document 0687
 DOCN  M9460687
 TI    Establishment of an MT4 cell line persistently producing infective HIV-1
       particles.
 DT    9404
 AU    Gyuris A; Vajda G; Foldes I; Microbiological Research Group, B. Johan
       National Institute of; Hygiene, Budapest, Hungary.
 SO    Acta Microbiol Hung. 1992;39(3-4):271-9. Unique Identifier : AIDSLINE
       MED/94160683
 AB    Human T cell Lymphotropic Virus-I (HTLV-I) carrying human T cell line
       MT4 is highly sensitive to Human Immunodeficiency Virus-1 (HIV-1). After
       HIV-1 infection cell clusters characteristic of intact MT4 rapidly
       disintegrate, syncytia appear and the cells die. Surviving MT4 cells
       were subcultured following HIV-1 infection of high multiplicity. We
       succeeded to establish an MT4 cell line continuously producing infective
       HIV (MT4/HIV-1). The original and the HIV-1 infected MT4 cells were
       morphologically similar. The MT4/HIV-1 cells proved to be nearly 100%
       positive in indirect immunofluorescence assay using the serum of an
       HIV-1 antibody positive individual. OKT4 surface antigen could not be
       demonstrated on MT4/HIV-1 cells. On electron microscopic pictures
       typical and atypical virus particles could be seen near the surface of
       the cell membrane. The persistently produced virus particles were
       infective for H9 and MT4 cells. The antigenic structure of the virus
       produced by MT4 cells was similar to that produced by H9 cells.
 DE    *Cell Line  Cell Line, Transformed  Cell
       Membrane/MICROBIOLOGY/ULTRASTRUCTURE  Comparative Study  Cytopathogenic
       Effect, Viral  Fluorescent Antibody Technique  Human
       HIV-1/IMMUNOLOGY/*PHYSIOLOGY/ULTRASTRUCTURE  HTLV-I  Microscopy,
       Electron  T4 Lymphocytes/*MICROBIOLOGY/ULTRASTRUCTURE  Virus Replication
       JOURNAL ARTICLE

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