Document 0690
 DOCN  M9460690
 TI    Human cytomegalovirus-mediated enhancement of human immunodeficiency
       virus type-1 production in monocyte-derived macrophages.
 DT    9404
 AU    Lathey JL; Spector DH; Spector SA; Department of Pediatrics, University
       of California, San Diego, La; Jolla 92393-0672.
 SO    Virology. 1994 Feb 15;199(1):98-104. Unique Identifier : AIDSLINE
       MED/94160585
 AB    Monocyte-derived macrophages were co-infected with human
       immunodeficiency virus (HIV), strain HIVBa-L, and human cytomegalovirus
       (HCMV) strain AD 169. HCMV enhanced the production of p24 antigen and
       infectious HIV, but HIV had no effect on HCMV production. In dually
       infected cultures HIV p24 antigen levels were increased 5- to 15-fold
       during the first 3 weeks of culture. This enhancement was observed both
       for cell-associated and extracellular p24 antigen from co-infected
       cells. The replication of macrophage-tropic clinical isolates of HIV
       were also enhanced by HCMV, but HCMV did not enable T cell-tropic
       viruses to replicate in macrophages. Conversely, HCMV clinical isolates
       from blood or urine were able to upregulate HIV production. Similar
       enhancement of HIV p24 antigen levels was observed with noninfectious,
       heat-inactivated HCMV, indicating that replication of HCMV in the
       macrophages was not necessary. Although replication was not required,
       the enhancing ability of HCMV was associated with the virus. Enhancement
       of HIV segregated with the HCMV pelleted virus particles and not the
       supernatant in stock virus. The fact that HCMV and HIV are detected in
       macrophages in persons with HIV infection highlights the importance of
       this system for studying interactions between HIV and HCMV in persons
       with HIV infection.
 DE    Cells, Cultured  Child, Preschool  Cytomegalovirus/GROWTH &
       DEVELOPMENT/*PHYSIOLOGY  Cytomegalovirus Infections/MICROBIOLOGY  Human
       HIV Core Protein p24/ANALYSIS  HIV Infections/MICROBIOLOGY  HIV-1/GROWTH
       & DEVELOPMENT/*PHYSIOLOGY  Interleukin-6/METABOLISM
       Macrophages/*MICROBIOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  Tumor Necrosis Factor/METABOLISM  Virus Replication  JOURNAL
       ARTICLE

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